破壁方法对雨生红球藻湿藻生物质中虾青素提取的影响  

The Effect of Cell-wall Disruption Methods on Astaxanthin Extraction from Wet Algal Biomass of Haematococcus pluvialis

作　　者：任晓丽 陈林[2] 刘天中[2] 李润植[3] Ren Xiaoli;Chen Lin;Liu Tianzhong;Li Runzhi(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong;Key Laboratory of Biofuel,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences, Qingdao 266101,Shandong;College of Agriculture,Shanxi Agricultural University,Jinzhong 030801,Shanxi)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]中国科学院青岛生物能源与过程研究所生物燃料重点实验室,山东青岛266101 [3]山西农业大学农学院,山西晋中030801

出　　处：《中国食品学报》2018年第12期110-117,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：山西省科技厅煤基科技攻关重点项目(FT-2014-01);中国科学院生物燃料重点实验室创新基金项目(CASKLB201503);中国科学院STS双创引导项目(KFJ-STS-SCYD-216)

摘　　要：为探讨破壁方法对雨生红球藻中虾青素提取的影响,以雨生红球藻湿藻细胞为原料,研究研磨、酸解、碱解、热蒸汽和酶解等多种破壁处理方法对细胞中的总类胡萝卜素和总脂肪的提取效率及其组成的影响。结果表明,酸解和酶解处理能够显著提高类胡萝卜素的提取效率,而不影响其组成。碱解处理导致酯化虾青素的水解,游离虾青素含量比未处理的对照组提高20倍,同时细胞内的甘油三酯也发生明显降解。热蒸汽和研磨处理对虾青素和油脂的提取率及其组成影响不明显。酶解和酸解是提高虾青素提取效率较有效的预处理方法,并能很好地保持虾青素和脂肪酸的结构稳定性和生物活性。The effect of cell-wall disruption methods on astaxanthin extraction from wet algal biomass of Haematococcus pluvialis was investigated.The contents and compositions of total carotenoids and total lipid were compared,after various treatments of cell-wall disruption,included grinding,enzymatic hydrolysis,acid hydrolysis,alkaline hydrolysis and hot steam.The results showed that the carotenoids extraction efficiencies were significantly increased,but their compositions were untouched,after either acid hydrolysis or enzymatic hydrolysis.The alkaline hydrolysis caused the degradation of esterified astaxanthin and increased the content of free astaxanthin to 20 folds of the control.Meanwhile,the intracellular triglyceride also significantly degraded.Hot steam and grinding had no obvious effect on the contents and compositions of the astaxanthin and lipid.In conclusion,enzymatic hydrolysis and acid hydrolysis,which maintained the stability and biological activity of the astaxanthin and the fatty acid,were the most effective pretreatment methods for cyst cells of H.pluvialis to improve the extraction efficiency of the astaxanthin.

关 键 词：雨生红球藻 虾青素 脂肪酸 破壁 提取率 

分 类 号：TQ28[化学工程—有机化工]