玉米黄粉酶解液在发酵生产黄原胶中的应用  

Application of Corn Gluten Meal hydrolysate in Xanthan Fermentation

作　　者：商峰 肖潇[1] 吴一平 尹胜[1] 王成涛[1] 卢松 任桂美 SHANG Feng;XIAO Xiao;WU Yi-ping;YIN Sheng;WANG Cheng-tao;LU Song;REN Gui-mei(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University,Beijing 100048;Neimenggu Fufeng B.iotechnologies Co.,Ltd.,Hohehot 010030,Inner Mongolia,China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心北京市食品添加剂工程技术研究中心北京工商大学,北京100048 [2]阜丰集团内蒙古阜丰生物科技有限公司,内蒙古呼和浩特010030

出　　处：《精细化工》2019年第1期51-56,共6页Fine Chemicals

基　　金：国家重点研发计划项目(2016YFD0400502;2018YFD0400403);国家自然科学基金项目(31571801);北京市科技创新服务能力建设-科技成果转化-提升计划项目(PXM2018-014213-000033);北京市科技计划项目(Z171100002217019)~~

摘　　要：以玉米黄粉酶解液为氮源,应用于黄原胶发酵培养基,探讨了蛋白酶种类、玉米黄粉酶解液添加量和酶添加量等对发酵生产黄原胶黏度和产胶率的影响,并优化了复配氮源的种类及复配比例。结果表明,碱性蛋白酶水解玉米黄粉的酶解液作为氮源发酵生产黄原胶的黏度和产胶率优于其他蛋白酶解液;当酶解液添加量为6%(以发酵培养液体积为基准,下同)、碱性蛋白酶添加量为6.0 kU/g时,发酵生产黄原胶的黏度和产胶率可以达到3235.7 mPa·s和24.4 g/L。相较于与棉籽粉、豆粕粉和花生粉复配,大豆蛋白与玉米黄粉酶解液复配发酵生产黄原胶的黏度和产胶率更高;玉米黄粉酶解液与大豆蛋白复配质量比为3∶7时,发酵黄原胶的黏度和产胶率可以达到5833.2 mPa·s和32.4 g/L,与单独以大豆蛋白为氮源相比无显著性差异。To apply enzymatic hydrolysate of corn gluten meal(CGM) as a nitrogen source for xanthan fermentation,the effects of protease type,enzymatic hydrolysate dosage and enzyme dosage on the viscosity and gum productive rate of xanthan were discussed.Furthermore,the nitrogen source combined with CGM hydrolysate and the ratio of CGM hydrolysate to soybean protein were optimized by single factor experiment.The results indicated that alkaline protease pretreatment had a better effect on the following xanthan fermentation than other proteases.The gum productive rate and viscosity of xanthan were 24.4 g/Land 3235.7 mPa·s when CGM dosage was 6%(based on the volume of fermentation medium,the same below) and alkaline protease dosage was 6.0 k U/g.The single factor experiments showed that soybean protein combined with CGM hydrolysate as a nitrogen source was better than cottonseed meal,soybean meal and peanut meal combined with CGM hydrolysate,respectively,when the mass ratio of CGM hydrolysate to soybean protein was 3∶7,the gum productive rate and viscosity of xanthan could reach 32.4 g/L and 5833.2 mPa·s,and there was no significant difference compared with that using soybean protein as a nitrogen source alone.

关 键 词：玉米黄粉 酶解液 黄原胶 氮源 产胶率 黏度 生物工程 

分 类 号：Q815[生物学—生物工程] Q919[天文地球—古生物学与地层学]