Role of using two-route ulinastatin injection to alleviate intestinal injury in septic rats  

作　　者：Xue-Lian Liao Qu-Zhen Danzeng Wei Zhang Chen-Shu Hou Bin-Bin Xu Jie Yang Yan Kang 

机构地区：[1]Department of Critical Care Medicine,West China Hospital,Sichuan Universzty,Chengdu 610041,China [2]Department of Critical Care Medicine,Tibet Autonomous Region People's Hospital,Lhasa 850000,Tibet,China [3]Department of Critical Care Medicine,Affiliated Hospital of Zunyi Medical College,Zunyi 563000,Guizhou Province,China

出　　处：《Chinese Journal of Traumatology》2018年第6期323-328,共6页中华创伤杂志（英文版）

基　　金：a grant from the National Natural Science Foundation of China (No.81701880).

摘　　要：Purpose:Early application of protease inhibitors through the intestinal lumen could increase survival following experimental shock by blocking the pancreatic digestive enzymes. Hence, it was hypothesized that two-route injection (intraintestinal +intravenous) of ulinastatin (UTI), a broad-spectrum protease inhibitor, could better alleviate intestinal injury than single-route injection (either intravenous or intraintestinal).Methods:A sepsis model induced by lipopolysaccharide on rats was established. The rats were randomly divided into five groups: sham, sepsis, UTI intravenous injection (Uiv), UTI intraintestinal injection (Uii), and UTI intraintestinal +intravenous injection (Uii +Uiv) groups. The mucosal barrier function, enzyme-blocking effect, levels of systemic inflammatory cytokines, and 5-day survival rate were compared among groups. The small intestinal villus height (VH), crypt depth (CD), and two components of mucosal barrier (E-cadherin and mucin-2) were measured to evaluate the mucosal barrier function. The levels of trypsin and neutrophil elastase (NE) in the intestine, serum, and vital organs were measured to determine the enzyme-blocking effect.Results:Compared with the single-route injection group (Uiv or Uii), the two-route injection (Uii +Uiv) group displayed: (1) significantly higher levels of VH, VH/CD, E-cadherin, and mucin-2;(2) decreased trypsin and NE levels in intestine, plasma, and vital organs;(3) reduced systemic inflammatory cytokine levels;and (4) improved survival of septic rats.Conclusion:Two-route UTI injection was superior to single-route injection in terms of alleviating intestinal injury, which might be explained by extensive blockade of proteases through different ways.

关 键 词：Sepsis ULINASTATIN INTESTINES TRYPSIN ELASTASE 

分 类 号：R[医药卫生]