A model for anticancer surveillance was pharmacologically developed to evaluate vitality principle in breast cancer rats  

作　　者：Liu Jinyu Zhang Mengyang Zhao Xin Ge Shasha Li Shuang Peng Lin Mu Yuxue Chen Chen Li Xiaoya Zhang Rui Feng Xuanye Deng Bo Jia Liqun Lin Yulin Wang Yueqi Cheng Zhiqiang TaeHoo Yi Cai Dayong 

机构地区：[1]Research Center for Pharmcology and Toxicology,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China [2]College of Basic Medicine,Beijing University of Chinese Medicine,Beijing 100029,China [3]Oncology Department of Chinese Medicine,China-Japan Friendship Hospital,Beijing 100029,China [4]College of Life Sciences,Kyung Hee University,Yongin-si,Gyeonggi-do 17104,Republic of Korea

出　　处：《Journal of Traditional Chinese Medicine》2018年第6期823-833,共11页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China"Via regulating ghrelin axis,Fuzi Lizhong pill effects on chronic atrophic gastritis with Spleen-Yang deficiency","Pharmacological mechanism of Yiqi-huoxue prescription on portal hypertension from inhibiting the oxidase hyperactivity of macrophages in portal triads"(No.81541082,81673674);the Beijing Natural Science Foundation"A pharmcodynamic model of systems biology for the joint actions of molecules from prescriptions with medical plants on portal hypertension"(No.7132150)

摘　　要：OBJECTIVE: To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.METHODS: The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene(DMBA, i.g.,100 mg/kg) at d_(001). The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant(CFA), the various intervals(k = 0.80) were dominated from d_(025)(600.00 h) to d_(095)(2288.82 h). The optimal surveillant status was confirmed with the median effective interval(EI_(50))from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis.The availability of this model was confirmed with Shugan Liangxue prescription(SLP), from the vitality principle, and assured further from interleukin-12 levels.RESULTS: The regressive curve was set up between the intervals and tumor volumes, the EI_(50) in SLP-treated rats(1475.00 h, Y_(SLP)= 0.1026 +0.8780/[1 + 10^(27.1425-8.565x)]) was postponed, which was 1.87 multiple of the EI_(50) in CFA rats(791.40 h, y =-0.0525 + 0.9452/[1 + 10^(30.4870-10.52x)], so did prepone the curve between the intervals and the immunological biomarker, serum interleukin-12 levels, the EI_(50) in SLP-treated rats(744.90 h, Y_(SLP)=-0.0145 + 0.7455/[1 + 10^(52.09636-18.13x)]) be 0.78 multiple of the EI_(50) in CFA rats(960.10 h, Y_(CFA)= 0.2460 + 0.7270/[1 + 10^(-67.1546 +22.52x)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.CONCLUSION: The model for anticancer surveillance was pharmacologically established as the optimal interval(791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available OBJECTIVE: To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.METHODS: The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene(DMBA, i.g.,100 mg/kg) at d_(001). The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant(CFA), the various intervals(k = 0.80) were dominated from d_(025)(600.00 h) to d_(095)(2288.82 h). The optimal surveillant status was confirmed with the median effective interval(EI_(50))from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis.The availability of this model was confirmed with Shugan Liangxue prescription(SLP), from the vitality principle, and assured further from interleukin-12 levels.RESULTS: The regressive curve was set up between the intervals and tumor volumes, the EI_(50) in SLP-treated rats(1475.00 h, Y_(SLP)= 0.1026 +0.8780/[1 + 10^(27.1425-8.565x)]) was postponed, which was 1.87 multiple of the EI_(50) in CFA rats(791.40 h, y =-0.0525 + 0.9452/[1 + 10^(30.4870-10.52x)], so did prepone the curve between the intervals and the immunological biomarker, serum interleukin-12 levels, the EI_(50) in SLP-treated rats(744.90 h, Y_(SLP)=-0.0145 + 0.7455/[1 + 10^(52.09636-18.13x)]) be 0.78 multiple of the EI_(50) in CFA rats(960.10 h, Y_(CFA)= 0.2460 + 0.7270/[1 + 10^(-67.1546 +22.52x)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.CONCLUSION: The model for anticancer surveillance was pharmacologically established as the optimal interval(791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available

关 键 词：BREAST cancer PHARMACOLOGY Drug screening assays antitumor Freund's ADJUVANT Shugan Liangxue prespriction 

分 类 号：R[医药卫生]