Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon  

作　　者：Song Pengfei Lin Zhigang Zhu Qingguang Jiang Shichao Zhang Hao Cheng Yanbin Sun Wuquan Wu Zhiwei Zhou Xin Lv Zhizhen Fang Min 

机构地区：[1]Department of Tuina,YueYang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [2]Fujian University of Traditional Chinese Medicine Subsidiary Rehabilitation Hospital,Fuzhou 350003,China [3]Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [4]Research Institute of Tuina,Shanghai Academy of Traditional Chinese Medicine,Shanghai 200437,China

出　　处：《Journal of Traditional Chinese Medicine》2018年第6期834-841,共8页中医杂志（英文版）

基　　金：Supported by High Level Innovation Team of Traditional Chinese Medicine Peak Plateau Subject(No.30304114316);Study on the Core Technical Specifications and Clinical Evaluation of Guided Exercise Therapy Combined with Acupoints Application in the Prevention of Chronic Fatigue Syndrome(No.2015468003-1);the National Natural Science Foundation of China(No.81603710);the National Natural Science Foundation of China(No.8150151117);Natural Science Foundation of Fujian Province(No.2017J01828);Major Disease Clinical Skills Upgrading Project of Shanghai Shen-kang Hospital Development Center(No.16CR1023A)

摘　　要：OBJECTIVE: To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.METHODS: A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight:(220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) ℃] and12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion(CCD), Tuina(7 d) and Tuina(21 d). CCD rat model was established via unilateral DRG compression by"L"liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold(PWT) and paw withdrawal latency(PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.RESULTS: There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference(P ≤ 0.05) between the groups.CONCLUSION: Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats.The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.OBJECTIVE: To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.METHODS: A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight:(220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) ℃] and12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion(CCD), Tuina(7 d) and Tuina(21 d). CCD rat model was established via unilateral DRG compression by"L"liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold(PWT) and paw withdrawal latency(PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.RESULTS: There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference(P ≤ 0.05) between the groups.CONCLUSION: Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats.The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.

关 键 词：TUINA GANGLIA SPINAL HYPERALGESIA Mechansensitiveion channels 

分 类 号：R[医药卫生]