MeCP2 deficiency promotes cell reprogramming by stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated ceil cycle gene translation  

作　　者：Wei Zhang Guihai Feng Libin Wang Fei Teng Liu Wang Wei Li Ying Zhang Qi Zhou 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Molecular Cell Biology》2018年第6期515-526,共12页分子细胞生物学报（英文版）

基　　金：the National Natural Science Foundation of China (31471395 to Q.Z.);the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY- SSW-SMCO02);the National Basic Research Program of China (2014CB964903).

摘　　要：The generation of induced pturipotent stem cells (iPSCs)offers a great opportunity in research and regenerative medicine.The current poor efficiency and incomplete mechanistic understanding of the reprogramming process hamper the clinical application of iPSCs. MeCP2 connects histone modification and DNA methyiation,which are key changes of somatic cell reprogramming.However,the rote of MeCP2 in celt reprogramming has not been examined.In this study,we found that MeCP2 deficiency enhanced reprogramming efficiency and stimulated cell proliferation through regulating cell cycle protein expression in the earLy stage of reprogramming.MeCP2 deficiency enhanced the expression of ribosomal protein genes,thereby enhancing reprogramming efficiency through promoting the translation of ceLl cycle genes.In the end,MeCP2 deficiency stimulated IGF1/AKT/mTOR signaling and activated ribosomal protein gene expression.Taken together,our data indicate that MeCP2 deficiency promoted cell reprogramming through stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated cell cycle gene translation in the early stage of reprogramming.

关 键 词：MECP2 CELL reprogramming IGFI/AKT/mTOR SIGNALING RIBOSOMAL protein CELL cycle 

分 类 号：Q[生物学]