微环境对脂肪酶催化乙酸甲基苯甲酯立体选择性氨解的影响  

EFFECT OF MICROENVIRENMENT ON LIPASE-CATALYZED ENANTIOSEL-ECTIVE AMMONOLYSIS OF (±) -α-METHYLBENZYL ACETATE

作　　者：李慧青[1] 宗敏华[1] 梁文峰[1] 何欢[1] 

机构地区：[1]华南理工大学生物工程系,广州510640

出　　处：《微生物学通报》2002年第1期35-40,共6页Microbiology China

基　　金：广东省自然科学基金资助项目(No.980543)

摘　　要：　系统研究了反应介质、水活度、温度、pH等因素对脂肪酶Novozym435催化乙酸甲基苯甲酯立体选择性氨解反应的影响。以正己烷为反应介质,酶表现出较高的催化活性和对映体选择性;适宜的反应温度为25℃～35℃;最适反应体系初始水活度为0.33;较适宜的pH范围为6～7。The effects of reaction media, water activity, temperature and pH on Novozym 435-catalyzed enantiose-lective ammonolysis of (±) -α-methylbenzyl acetate have been systematically explored. Novozym 435 showed high catalytic activity and enantioselectivity in hexane; the optimum temperature and the initial water activity were 25℃ and 0.33 respectively; The suitable reaction pH was in the range of 6.0 - 7.0.

关 键 词：催化 微环境 脂肪酶 乙酸甲基苯甲酯 立体选择性氨解 

分 类 号：O643.3[理学—物理化学]