上皮性卵巢癌中nm23-H1基因突变  

Genomic PCR-SSCP analysis of the mutation of nm23-H1 gene:a study on ovarian carcinoma

作　　者：王忠民[1] 常德安[2] 

机构地区：[1]大连市妇产医院,大连116031 [2]大连医科大学附属第二医院妇产科

出　　处：《肿瘤》2002年第3期209-211,共3页Tumor

摘　　要：目的　本文研究nm2 3 H1基因在上皮性卵巢肿瘤中的突变情况 ,以期寻找监测卵巢癌转移并能指导治疗的指标。方法　利用非同位素PCR SSCP技术研究nm2 3 H1基因的 5个外显子的突变情况。结果　正常卵巢、良性卵巢癌及交界性瘤均未发现突变 ,卵巢癌突变率显著增高。低分化程度卵巢癌突变率高于高、中分化程度的卵巢癌。浆液性卵巢癌nm2 3 H1基因突变率高于其他组织学类型。Ⅲ、Ⅳ期卵巢癌突变率高于Ⅰ、Ⅱ期卵巢癌。发生淋巴结转移的卵巢癌突变率高于无淋巴结转移的卵巢癌。结果　nm2 3 H1基因突变容易发生于分化程度较低、有淋巴结转移的晚期卵巢癌病例中。Objective The mutation of a putative metastatic suppressor gene, nm23 H1 gene was studied looking for an index for monitoring mutation and instructing treatment of ovarian carcinoma. Method Polymerase chain reaction (PCR) single strand conformational polymorphism (SSCP) analysis was performed to examine the mutation in 5 exon of nm23 H1 gene. Results Mutation was found in 9 out of 32 cases of malignant ovarian tumors (28.1%), while no mutation was found in normal ovaries, bengin ovarian tumors and borderline ovarian tumors. The rate of mutation in malignant cases was significantly higher than other three types ( P <0 05) The rate of mutation of nm23 H1 gene in grade Ⅲ was higher than in grade Ⅰ,Ⅱ( P <0 005) Among ovarian carcinomas with different histological types, mutation in serous carcinoma was higher than in mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma. Among different clinical stages, the rate of mutation of nm23 H1 gene in stage Ⅲ、Ⅳ was higher than in stage Ⅰ、Ⅱ ( P <0 05). The rate of mutation in cases with lymph node metastases was higher than in cases without metastases ( P <0 05). Conclusion These results suggested that mutation of nm23 H1 gene tends to exist in advanced serous ovarian carcinoma with lymph node mestatases and less differentiated tissues.

关 键 词：上皮性卵巢癌 NM23-H1基因 基因突变 聚合酶链反应-单链构象多态性 

分 类 号：R737.31[医药卫生—肿瘤]