人工制备黄曲霉毒素B_1抗原的研究  

PVEPARATION OF AFLATOXIN B_1 1 MMUNOGEN

作　　者：张坐奎[1] 余礼碧[2] 张伯玲[2] 陈宁蓉 

机构地区：[1]四川大学原子核科学技术研究所,成都610064 [2]成都军区军事医学研究所,成都610061

出　　处：《西南农业学报》1991年第4期115-118,共4页Southwest China Journal of Agricultural Sciences

摘　　要：在AFB_1分子上引入羧甲氧基氨,将其转化成AFB_1肟,用硅胶H柱色层层析对转化物进行分离,并用红外光谱、质谱鉴定,AFB_1肟的收率为理论产率的89%.并用碳二亚胺法将AFB_1肟成功地偶联到BSA上制成人工抗原AFB_1-oxime-BSA,研究了溶剂对偶联作用的影响.结果表明DMA-水体系比乙醇-水省时、省试剂.An aflatoxin B1-l-(O-Carboxy methyl) Oxime uas prepared from aflatoxin B1 and purified. The overall yield was about 89%. The new aflatoxin B1 derive was characterized by mass spectrum, ultraviolet and infrared spectral analyses. An artificial immunogen, aflatoxin B1-ome-BSA Conjugate, with a molecular ratio 16:1 of hepten to carries protein, was synthesized. The effect of different solvents for coupling aflatoxin B1-Oxime with BSA was studied and show to be advantageous in DMF-water.

关 键 词：黄曲霉 毒素B1 抗原 制备 

分 类 号：S432.44[农业科学—植物病理学]