灰树花胞外多糖的性质与结构  

The Character and Structure of Extracellular Polysaccharide from Grifola frondosa

作　　者：陈石良[1] 马青[2] 谷文英[1] 陶文沂[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036 [2]杭州娃哈哈集团有限公司科研中心,浙江杭州310020

出　　处：《无锡轻工大学学报（食品与生物技术）》2002年第3期244-248,共5页Journal of Wuxi University of Light Industry

摘　　要：对发酵法生产的灰树花胞外多糖GLP A 2的理化特性与结构进行了初步研究 .经凝胶柱层析和HPLC检测纯度 ,证明水溶性多糖GLP A 2为单一均匀组分 ,不含蛋白质和核酸 ,比旋光度[α]12D =+ 177.5 0°(H2 O ,0 .1) ,HPLC法测得平均相对分子质量为 6 .2× 10 4 .GC、IR和13C NMR分析表明 ,GLP A 2是一类 β 葡聚糖 ,分子的主链以 β (1→ 3) 糖苷键连接 ,并含有 β (1→ 6 )、α (1→2 )、α (1→ 3)及 β (1→ 2 )糖苷键相连的侧链 .GLP A 2可与刚果红结合 ,形成的络合物在 0～ 0 .4mol/L的NaOH溶液中 ,表现出最大吸收波长 (λmax)的特征变化 ,同时在CD谱中出现明显的有序结构信息 .这提示GLP A 2在溶液中存在三股螺旋构象 。The physical and chemical characteristics of GLP A 2 isolated from fermentation broth of Grifola frondosa were primarily determined. The water soluble polysaccharide GLP A 2 was proved to be a homogeneous component by using the techniques of Sephadex G 200 colum chromatography and HPLC. It had a optical rotation D= +177.50°(H 2O,0.1).The average molecular weight of GLP A 2 was 6.2×10 4 determined by HPLC. The structure of GLP A 2 was determined by GC,IR and 13 C NMR. The results showed that GLP A 2 was β D glucan ,which possessed a β (1→3) linkaged backbone and sidechains involving β (1→6), β(1→2),α (1→2), α(1→3) glycosidic bonds. GLP A 2 could form a complex with Congo red and exhibited the specific change in the absorption maximum of the Congo Red glucan complex. On the other hand, CD spectra analysis showed that GLP A 2 had an ordered molecular structure. All these indicated that GLP A 2 probably took a triple helical conformation in water and its hydrogen bond force was probably a main force supporting that conformation.

关 键 词：灰树花 胞外多糖 性质 结构 抗肿瘤 免疫功能 

分 类 号：O636[理学—高分子化学]