胃癌中p16基因缺失与突变的研究  

Analysis of p16 gene deletion and mutation in gastric carcinoma

作　　者：赵国海[1] 李铁臣[2] 史良会[1] 夏亚斌[1] 孙惠兰[2] 彭敦发[3] 

机构地区：[1]皖南医学院附属弋矶山医院外科,安徽芜湖241001 [2]皖南医学院生物学教研室,安徽芜湖241001 [3]皖南医学院病理学教研室,安徽芜湖241001

出　　处：《中国病理生理杂志》2002年第5期543-545,共3页Chinese Journal of Pathophysiology

基　　金：安徽省教委自然科学基金资助 (No .99jl0 2 16 )

摘　　要：目的 :调查胃癌患者p16基因的改变。方法 :采用PCR和DNA测序方法 ,对 36例胃癌患者肿瘤组织标本的p16基因外显子 1、2和 3进行检测 ,研究p16基因的缺失和突变情况。结果 :发现 2例有p16基因外显子 1的纯合缺失 ,3例有外显子 3的纯合缺失 ,缺失频率为 13 89% ,5例均为弥漫型胃癌。所有标本未检出点突变。结论AIM: To investigate p16 gene alterations in gastric carcinoma. METHODS: 36 fresh tumor specimens taken from gastric cancer patients were analyzed for p16 gene deletion and mutation by polymerase chain reaction (PCR) and DNA sequencing. RESULTS: Homozygous deletion of exon 1 and exon 3 was observed in 2 cases and 3 cases of diffuse carcinoma, respectively. The frequency of homozygous deletion was 13.89%. No p16 gene point mutation was detected. CONCLUSION: The deletion of p16 genes may be related to gastric carcinogenesis.

关 键 词：胃癌 P16基因 纯合缺失 基因突变 

分 类 号：R735.2[医药卫生—肿瘤]