位点特异性重组酶Cre的克隆、表达、纯化及体外活性鉴定  

Cloning Expression Purification and Biological Activity of Site Specific Recombinase Cre

作　　者：高虹[1] 张传生[1] 魏影允[1] 胡国法[1] 劳为德[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100080

出　　处：《高技术通讯》2002年第6期31-33,共3页Chinese High Technology Letters

基　　金：中国科学院知识创新工程 (KSCX1 0 5 0 1)项目资助

摘　　要：克隆了Cre基因 ,分别构建了真核表达载体 pEF EBFP Cre和 pGEX Cre,在大肠杆菌中表达了Cre重组酶 ,并证明了重组酶的删除特性 。Cre/loxP system is the powerful tool for the study of gene expression and control. We construct the eukaryotic expression vector pEF EBFP Cre and prokaryotic expression vector pGEX Cre. The Cre recombinase is expressed in E. coli and its deletion property is observed. The expressed Cre recobinase could be used in the further transgenic mammary bioreactor research.

关 键 词：位点特异性重组酶Cre 克隆 纯化 体外活性 鉴定 CRE/LOXP系统 原核表达 CRE基因 基因表达 大肠杆菌 

分 类 号：Q785[生物学—分子生物学]