白腐真菌AH28-2菌株发酵合成漆酶初步研究  

PRELIMINARY STUDIES ON LACCASE PRODUCTION BY A WHITE-ROT FUNGUS AH28-2

作　　者：张敏[1] 肖亚中[1] 蒲春雷 赵萍 王军[1] 吴涓[1] 王怡平[1] 

机构地区：[1]安徽大学生命科学学院,合肥230039

出　　处：《微生物学通报》2002年第3期37-42,共6页Microbiology China

基　　金：安徽省自然科学基金资助项目 (No. 982 1 2 4 1 1 );安徽省高校自然基金重点资助项目 (No. 2 0 0 0J1 0 1 3)

摘　　要：从 2 2 4个野外采集的真菌样品中筛选分离到一株产漆酶活性较高菌株AH2 8 2 ,经初步鉴定为白腐真菌。采用单因子相互比较法 ,研究了该菌株最适发酵产酶条件。应用添加有 1g/LKraft木素的液体发酵培养基 ,接种量 5 %(V/V) ,初始pH8 5 ,装液量为 5 0 %,2 8℃、 1 5 0r/min摇瓶振荡培养 4～ 5d ,漆酶酶活水平达 2 0 1 84IU/L。A novel white-rot fungus AH28-2,which was isolated from 224 fungi samples,ability to produce effectively laccase by induction.Several factors influencing laccase production were investigated.The optimum conditions were as follows:the 300mL Erlenmeyer flask containing 150mL of liquid medium was inoculated with 7.5mL of mycelial fragments and the medium was supplied with lignin at a concentration of 0.1%(initial pH8.5).The cultures was incubated at 28℃ on rotary shaker(150r/min) for 4～5 days.The maximum enzyme activity was 20184IU/L.

关 键 词：白腐真菌 AH28-2菌株 发酵 合成 漆酶 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q949.32[生物学—植物学]