前列腺癌与雄激素受体基因(CAG)n重复多态性的关系  

Study on the polymorphism of (CAG)n repeats within androgen receptor gene in patients with prostate cancer

作　　者：王钢[1] 陈光椿[1] 王晓慧[1] 夏冰[1] 张金山[1] 卢建[1] 

机构地区：[1]第二军医大学病理生理学教研室,上海200433

出　　处：《中华泌尿外科杂志》2002年第6期351-353,共3页Chinese Journal of Urology

基　　金：国家自然科学基金 (3 9670 3 0 0 )

摘　　要：目的　探讨前列腺癌 (PC)与雄激素受体 (AR)基因 (CAG)n重复多态性的关系。　方法　应用DNA双链循环测序方法对 34例PC组织与癌旁正常组织、2例PC患者外周血白细胞内的AR基因 (CAG)n重复数进行测定。　结果　同一PC患者的癌组织与癌旁正常组织 (CAG)n重复数相同 ;36例患者癌组织AR基因 (CAG)n呈重复多态性 ,平均 2 0 .0 6 ,显著低于正常组织 ,差别有显著性意义 (P <0 .0 5 ) ;不同分化程度的癌组织 (CAG)n重复数差别无显著性意义 (P >0 .0 5 )。　结论　AR基因 (CAG)n重复数改变的体细胞突变在PC癌细胞中罕见 。Objective To study the relationship between polymorphic (CAG)n repeats of androgen receptor(AR) gene and occurrence of prostate cancer (PC). Methods (CAG)n repeats of both malignant cells and adjacent nonmalignant cells from 34 paraffin embedded PCs, and that of peripheral blood cells from 2 PC patients were assessed using dsDNA cycle sequencing. Results The number of (CAG)n repeats in malignant cells was equal to that in adjacent nonmalignant cells from the same paraffin embedded PC; the mean of (CAG)n repeats in the 36 PC patients was 20.06 which was significantly smaller than that of normal men ( P <0.05), although no significant difference in the polymorphic distribution of (CAG)n was found among various grades of PC( P >0.05). Conclusions Shortening of (CAG)n repeats within AR gene may be associated with the occurrence of PC.

关 键 词：前列腺癌 雄激素受体基因 重复多态性 前列腺肿瘤 (CAG)n基因 体细胞突变 PC 

分 类 号：R737.25[医药卫生—肿瘤]