响应面法优化米曲霉HML366产β-葡萄糖苷酶发酵培养基  

Optimization of fermenting culture medium for producing β–glucosidase by Aspergillus oryzae HML366 with response surface methodology

作　　者：罗奉奉[1] 何海燕[1] 

机构地区：[1]河池学院化学与生物工程学院,广西宜州546300

出　　处：《大众科技》2014年第6期98-100,共3页Popular Science & Technology

基　　金：广西理工科学实验中心开放课题:微生物降解木质纤维素及新纤维素酶纯化鉴定研究(LGZXKF201109);河池学院重点课题:蚕沙发酵产果胶酶的菌种选育及酶学性质研究(2013JZA-H002)

摘　　要：文章研究旨在优化前期筛选到的米曲霉HML366产β-葡萄糖苷酶的培养基条件。通过单因素实验和响应面设计对米曲霉HML366产β-葡萄糖苷酶的培养基条件进行了优化组合,确定最佳的发酵培养基配方为:甘蔗渣3.49%,豆饼粉0.375%,KH2PO4 0.255%。在此培养条件下得到β-葡萄糖苷酶酶活力达92.64 U/g,是未优化前酶活71.12 U/g的1.30倍。The study was to optimize the medium conditions ofβ-glucosidase production by preliminary screening Aspergillusoryzae HML366. Optimization of fermenting culture medium for producingβ-glucosidase by AspergillusoryzaeHML366 with single factor test and response surface methodology, the optimum components of mediums consisted of bagasse 3.49%, bean cake powder 0.375%, KH2PO4 0.255%. Under this condition theβ-glucosidase activity was up to 92.64U/g, it was 1.30 times compared with the activity 71.12 U/g before optimized.

关 键 词：米曲霉 Β-葡萄糖苷酶 优化 响应面 

分 类 号：TQ92[轻工技术与工程—发酵工程]