水稻纹枯病菌拮抗菌株F_2的抗菌活性  

Antimicrobial Activity of An Antagonistic Strain F_2 Against Thanatephorus cucumeris

作　　者：何爱莲[1,2] 雷帮星[1] 康冀川[1] 郭坤亮[2] 何劲[3] 文庭池[1,2] 

机构地区：[1]贵州大学教育部西南药用生物资源工程研究中心,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵阳学院,贵州贵阳550005

出　　处：《贵州农业科学》2014年第6期82-86,共5页Guizhou Agricultural Sciences

基　　金：贵州省科技厅创新能力建设项目"生物活性成分智能化中试提取平台建设及在产品开发中的应用"[黔科合G(2010)4009];贵州省烟草总公司科技项目"贵州植烟土壤微生物修复关键技术的研究与应用"(201018);贵州省"十二五"公益性行业(农业)科研专项"利用有机(类)肥料调控我国土壤微生物区系关键技术研究"(201103004)

摘　　要：为探明拮抗菌株F2对水稻纹枯病菌的抗菌活性，采用菌饼法，研究了水稻纹枯病菌拮抗菌株F2的抗菌活性。结果表明：F2菌株发酵滤液中抗菌物质在5-7d 内产生较快，7d 时发酵滤液对水稻纹枯病菌抑菌率达62．5％；随着 F2菌株发酵滤液浓度的增加，其对水稻纹枯病菌的抑菌率呈上升趋势，当发酵滤液浓度为30％时，其对水稻纹枯病菌的抑菌率为74．27％，与0．75％的百菌清（76．47％）相当；PD 培养液发酵培养 F2菌株，其发酵滤液对水稻纹枯病菌的抑菌率最高，其余依次为牛肉膏蛋白胨培养液、LB 培养液、基础培养液和豆芽汁培养液；发酵滤液中抗菌物质对不同酸碱度、紫外线和储存时间的稳定性强，且热稳定性较好。Antifungal activity of antagonistic strain F2 on T .cucumeris was studied by using fungus plugs method. The results showed that the rapid production period of antifungal substances in F2 fermentation filtrate were 5-7 d,and the 7 d reached the maximum inhibition rate of fermentation filtrate, 62.5% ,which positively correlated with concentration of the fermentation filtrate.The antifungal rate of 30% fermentation filtrate was 74.27%,which was similar to the effect in the 0.75% Chlorothalonil （76. 47%）.Anti T.cucumeris rate of F2 fermentation filtrate reached the peak when the filtrate was derived from PD broth,followed by beef extract peptone,LB,basis and bean sprouts juice broth.Antifungal substances in fermentation filtrate had good stability to pH,UV,storage time and temperature.

关 键 词：F2 枯草芽孢杆菌 水稻纹枯病菌 抑菌活性 稳定性 

分 类 号：S435[农业科学—农业昆虫与害虫防治]