酶解白肛海地瓜蛋白制备抗氧化肽的工艺研究  

Optimization of enzymatic hydrolysis parameters for antioxidant capacity of peptide from sea cucumber(Acaudina leucoprocta)

作　　者：袁坤山[1] 孙国琼[2] 郑艺[1] 于跃芹[1] 王雷[2] 王宝杰[2] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042 [2]中国科学院海洋研究所生物技术中心,青岛266071

出　　处：《食品科技》2014年第7期76-83,共8页Food Science and Technology

基　　金：国家自然科学基金项目(20876081)

摘　　要：为了能够有效利用低值的白肛海地瓜,以水解度为主要指标,对酶解蛋白酶进行了筛选,并系统研究了酶解温度、pH、加酶量和水解时间等单因素对水解度的影响。在此基础上,利用响应面中心组合设计实验,对酶解工艺进行了优化,得到以温度、pH、加酶量及水解时间为因子的二次方程,通过方差分析和验证性实验得出,此二次方程较能反应海参内脏团蛋白水解度的变化规律,得到最佳水解条件为:温度60℃,pH7.6,加酶量2.5%(质量比),酶解时间3.8 h,预测最高水解度为52.44%。最佳酶解条件下所得多肽73.08%以上分子量小于500 u。利用DPPH法测定最佳条件酶解所得多肽的抗氧化活性,3 mg/mL的抗氧化率达到了20.6%,利用ABTS法测定最佳条件酶解所得多肽的抗氧化活性,3 mg/mL的抗氧化率达到了0.203μmol/g。为海参内脏蛋白的利用提供了参考。In order to increase the utilization value of sea cucumber （Acaudina leucoprocta） as a potential source of peptides, protamex was selected for the hydrolysis of sea cucumber. One-factor-at-a-time method was used to investigate the effects of temperature, pH, enzyme dosage, and hydrolysis time on hydrolysis degree. Following these investigation, a quadratic regression model describing hydrolysis degree as a response to s temperature, pH, enzyme dosage, and hydrolysis time was set up using a Box-Behnken experimental design. As demonstrated by the results analysis of variance and validation experiments, the model could well indicate the hydrolysis degree of sea cucumber. The optimal hydrolysis conditions of temperature, pH, enzyme dosage, and hydrolysis time were determined to be 60 ℃, 7.6, 2.5% （dry mass ratio） and 3.8 hours, respectively. Under these conditions, the hydrolysis degree of sea cucumber is 52.44%. Under the best conditions of enzymatic hydrolysis, more than 73.08% of the the peptide is less than 500 u. It measured the antioxidant activity of peptides by DPPH method, showing radical scavenging activity is 20.6% when the concentration about 3 mg/mL. It measured the antioxidant activity of peptides by ABTS method, showing the Vc Equivalent Antioxidant Capacity is 0.203 μmol/g when the concentration about 3 mg/mL.

关 键 词：酶解 白肛海地瓜 响应面分析 蛋白酶 

分 类 号：S255.3[农业科学—农业机械化工程]