11种花粉水提物体外抗氧化能力研究  

Antioxidant activity of water extracts from eleven kinds of pollen

作　　者：王雪飞[1,2] 张华[1] 王振宇[1] 杜宇虹[2] 白海娜[1] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090 [2]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150086

出　　处：《食品工业科技》2014年第15期139-143,共5页Science and Technology of Food Industry

摘　　要：本文以11种花粉为研究对象，采用清除ABTS＋、·OH及总还原力三个体外指标来评价花粉水提物的抗氧化能力。并对水提物中生物活性物质蛋白质、多糖、多酚及黄酮含量进行测定。通过研究发现，11种花粉对ABTS＋，·OH均具有清除能力，但清除能力存在差异（P〈0．05），总还原力之间存在差异（P〈0．05）。三项抗氧化能力指标分析显示，具有较强抗氧化能力的花粉分别是黄柏花粉、玉米花粉及珍珠梅花粉。成分分析显示，11种花粉水提物中多糖含量较高，在49～145mg,／g之间，其次是多酚含量，在11-21mg／g之间，相关性分析显示，多糖和多酚含量对总还原力有显著影响（P〈0．05），多酚含量对清除ABTS＋的能力也有显著性影响（P〈0．05）。The antioxidant activities of different pollen water extracts were evaluated by ABTS ＋ scavenging method, · OH scavenging capability method and total reducing ability method. The total content of protein, polysaccharide, polyphenols and flavonoids were evaluated. Results indicated that eleven kinds of pollen extracts had strong scavenging effect on ABTS ＋ and ·OH respectively. Moreover,their scavenging activities against ABTS ＋ and ·H revealed a significant difference （ p 〈 0.05 ）. The significant difference （ p 〈 0.05 ） were also existed in the total reducing ability.Three antioxidant capacity indicated that cortex phellodendri pollen, corn pollen and pearl plum pollen had stronger antioxidant capacity than others. Through determining bioactive components, higher polysaccharide content were discovered with values of 49- 145mg/g, followed by polyphenols contents with 11 -21mg/g. Correlation analysis showed that the polysaccharide and polyphenol had a significant influence on the total reducing ability（p 〈 0.05）, polyphenol content also had a significant effect on scavenging ABTS ＋ （p 〈 0.05）.

关 键 词：花粉 抗氧化 多糖 多酚 

分 类 号：TS201.2[轻工技术与工程—食品科学]