响应面法优化荚果蕨总三萜超声提取工艺  

Optimization of ultrasonic-assisted extraction of total triterpenes from Matteuccia struthiopteris by response surface methodology

作　　者：杨晓艳[1,2] 马骥[1,2] 彭飞[1,2] 陆洋[1,2] 马吉福[1,2] 习伟佳 张婷[1,2] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]教育部药用植物资源及天然药物化学重点实验室,陕西西安710062

出　　处：《食品工业科技》2014年第15期200-204,209,共6页Science and Technology of Food Industry

基　　金：国家"十二五"科技支撑计划课题(2011BAI06B05);国家大学生创新实验项目(201310718050)

摘　　要：以荚果蕨为研究对象,在单因素实验的基础上,选取乙醇浓度、料液比、超声温度、超声时间为自变量,荚果蕨中总三萜的提取率为响应值,依据Box-Benhnken原理设计四因素三水平实验。通过回归分析得到最优提取工艺参数为:乙醇浓度60%,料液比1∶35,超声温度63℃,超声时间39min。总三萜提取率理论值为35.7263mg/g,实际值为36.0304mg/g,相对标准偏差为0.40%,表明该优化工艺稳定可靠,可为后续实验提供一定的理论指导和参考依据。Matteuccia struthiopteris was regarded as study material. According to the center combination （Box- Benhnken）experimental principles,four factors and three levels which were based on single factor experiments were selected to design response surface test. The response factor was the yield of triterpenes, independent variables were including ethanol concentration, solid- liquid ratio, ultrasonic temperature, and ultrasonic time. By means of regression analysis,the results showed that the optimum technological method to extract triterpenes from Matteuccia struthiopteris were as follows： ethanol concentration 60%, ratio of solid - liquid 1 ： 35, ultrasonic temperature 63% and ultrasonic time 39min. The actual value was 36.0304mg/g, compared with the theoretical value（35.？263mg/g） ,the RSD was 0.40%.From this study, it could be concluded that the extraction system was reliable and practical, the certain reference and theoretical guidance for further research can be provided.

关 键 词：荚果蕨 总三萜 超声 响应面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]