藏灵菇发酵乳乳清的抗氧化及免疫学活性研究  

Antioxidant and Immunological Activity of Whey Isolated from Tibetan Kefir Milk

作　　者：屈长青[1,2] 杜召凤[1] 韦兵[1] 周华艳[1] 姬云涛[1] 

机构地区：[1]阜阳师范学院生命科学学院 [2]抗衰老中草药安徽省工程技术研究中心,阜阳236041

出　　处：《天然产物研究与开发》2014年第7期1117-1119,共3页Natural Product Research and Development

基　　金：国家自然科学基金项目(31172182;31201788);安徽省高校自然基金项目(KJ2012Z315;KJ2013A206);安徽省省级科研平台委托专项项目(2013KSLZX04;2014KSLZX03)

摘　　要：藏灵菇培养发酵后,将发酵液加热除去沉淀,获得乳清液,冷冻干燥后得到乳清粉,将其分别配成1%和5%溶液进行小鼠灌胃实验,15 d后,检测昆明种小白鼠力竭游泳时间、血液SOD活性、对鸡红细胞致敏小鼠血清溶血素水平、小鼠腹腔巨噬细胞吞噬活性及对二硝基氟苯诱导小鼠迟发型超敏反应的影响。结果表明:5%剂量组的小鼠力竭游泳时间明显延长、红细胞中SOD的活性也明显提高、并能提高血清溶血素水平(P<0.05);而且1%和5%剂量组都具有提高腹腔巨噬细胞吞噬率的作用(P<0.05),这为开发利用藏灵菇提供了新的思路。In this study, the Tibetan kefir was cultured, fermented and was then heated and removed the precipitate to ob- tain whey. Twenty-four mice were selected and randomly divided into three groups, namely 1% whey group, 5% whey group and blank control group, to perform the corresponding treatments for 15 consecutive days. At the end of administra- tion, the swimming tests were carried out. The fatigue time was observed. The blood SOD, the serum hemolysin level and the phagocytosis of celiac macrophage were detected. It was found that the exhaustive swimming time of the experimental groups were significantly longer than that of blank control group （P 〈 0.05 ）. Compared to blank control group,the con- tent of SOD in mice of the experimental groups were significantly increased （P 〈 0.05）, the serum hemolysin level and the ability of phagocytosis of celiac macrophage were enhanced. These results showed that the whey isolated from Tibetan kefir milk sigui/icantly increased the activity of antioxidant enzymes in mice. These results will provide a new way of thinking for the development and utilization of Tibetan kefir.

关 键 词：藏灵菇 乳清 抗氧化 免疫学活性 

分 类 号：R963[医药卫生—微生物与生化药学]