高效液相色谱法同时测定面粉及面粉改良剂中的曲酸和噻二唑  

Simultaneous determination of kojic acid and 2-mercapto-5-methyl-1,3,4-thiadiazole in flour and flour improver

作　　者：简龙海[1] 钟吉强[1] 王欣美[1] 郑荣[1] 刘畅[1] 王柯[1] 

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《中国食品卫生杂志》2014年第4期354-357,共4页Chinese Journal of Food Hygiene

摘　　要：目的建立高效液相色谱法（HPLC）同时测定面粉及面粉改良剂中的曲酸和噻二唑。方法色谱柱AtlantisC18（4．6mm×15cm，5μm），流动相组成为乙腈一0．1％磷酸溶液，梯度洗脱，流速1．0ml／min，进样体积1μl。曲酸和噻二唑的检测波长分别为269和300nm。样品以乙腈提取，提取液经离心、滤过后供HPLC分析。结果曲酸、噻二唑均在0．5～20μg／ml范围内线性关系良好，平均加标回收率为84．2％～92．4％，RSD为1．1％～6．9％（n=6），检出限（LOD）分别为2．0和0．7mg／kg。结论该方法可用于面粉和面粉改良剂中曲酸和噻二唑的测定。Objective To establish a method for simultaneous determination of kojic acid and 2-mercapto-5-methyl-1, 3, 4-thiadiazole （MMTD） in flour and flour improver by HPLC. Methods Separation was achieved on an Atlantis C18 column （4. 6 mm ×15 cm, 5 μm） with the mobile phase of acetonitrile and 0.1% phosphoric acid solution. Gradient elution was used with the flow rate of 1.0 ml/min. The injection volume was 1 μl and the detection wavelength was 269 nm for kojic acid and 300 nm for MMTD. Kojic acid and MMTD in samples were extracted by acetonitrile. After centrifugation and filtering, sample solutions were analyzed by HPLC. Results Kojic acid and MMTD both showed good linearity in the range of 0. 5-20 μg/ml. The average recoveries were in the range of 84.2% -92.4% （RSD 1.1% -6. 9% , n =6）. Limit of detection （LOD） of koiic acid and MMTD were 2.0 and 0.7 mg/kg, respectively. Conclusion The method can be usedfor the determination of kojic acid and MMTD in flour and flour improver.

关 键 词：曲酸 噻二唑 面粉 面粉改良剂 高效液相色谱法 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]