高效薄层色谱法同时快速检测饮料中26种人工色素的研究  

Rapid and Simultaneous Detection 26 Kinds of Artificial Colors in Beverage by High Performance Thin Layer Chromatograph

作　　者：张兰天 王红[1,2] 王利军 匡林鹤 张会军 付晓华[2] 

机构地区：[1]河北省食品质量监督检验研究院,石家庄050091 [2]河北师范大学,石家庄050016

出　　处：《食品工业》2014年第8期238-242,共5页The Food Industry

基　　金：河北省科学技术研究与发展计划项目(11277163)

摘　　要：利用高效薄层色谱技术建立了检测饮料中26种人工色素的快速定性和定量分析方法。饮料样品1︰1稀释后直接在高效薄层铝板点样展开,通过颜色和Rf与26种人工色素标准薄层色谱板对比定性,薄层色谱扫描仪定量。该方法无需前处理,能够快速定性,准确定量,实现饮料产品的大批量检测,26种人工色素的回收率在82%~103%之间,RSD在0.9%~12.7%之间。The rapid detection method for 26 kinds of qualitative and quantitative artificial colors in beverage was established by high performance thin layer chromatograph. The samples of beverage were diluted and spotted on the aluminium plate, and Were spread by spread solvent The qualitative detection was completed through Rfvalue and color which were contrasted with the standard plate of 26 kinds of artificial colors, and the quantitative were completed by the instrument ofthrough high performance thin layer chromatograph. This method was qualitative rapidly and quantitative accurately without pre-processing, which could be realized batch detection for beverages and the value of recovery in the range of 82%-103%, and RSD were 0.9%-12.7%.

关 键 词：高效薄层色谱 人工色素 快速检测 

分 类 号：O657.7[理学—分析化学] TS27[理学—化学]