超高效液相色谱串联质谱法测定腐竹中乌洛托品的含量  

Determination of Urotropine in dried beancurd sticks by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：吴颖[1] 张慧[1] 崔芳[1] 姜洁 

机构地区：[1]北京市产品质量监督检验院,北京101300 [2]北京市食品安全监控和风险评估中心,北京100041

出　　处：《食品工业科技》2014年第17期298-300,共3页Science and Technology of Food Industry

摘　　要：建立用超高效液相色谱-串联质谱法（UPLC—MS／MS）测定腐竹中乌洛托品含量的方法。用乙腈提取腐竹中的乌洛托品，经固相萃取柱PXA净化，用UPLC—MS／MS分析，采用多反应监测模式，外标法定量。采用ACQUITYUPLCBEHHILIC色谱柱，以乙酸铵和乙腈为流动相，进行梯度洗脱。结果表明：该方法标准曲线良好，线性范围为1~20μg／L，最低检出限为1μg/L，三个水平添加浓度的平均回收率范围为81．67％-101．67％。本方法操作简单快捷，方法准确度和稳定性好。A method for the determination of urotropine in dried beancurd sticks was established by UPLC-MS/MS. The sample was extracted with acetonitrile as extraction solvents, then further purified by PXA solid phase extraction column.The analyte was determined by multi-reaction monitoring （MRM）mode was employed for the quantitative determination,and quantified by the external standard curve.The separation was performed on a UPLC ACQUITY BEH HILIC column by gradient elution with a system of water（containing 10mmol/L ammonium acetate buffer）-acetonitrile as mobile phase.Results showed that the calibration curve had good linearity.The developed method exhibited good linearity over the range from t to 20μg/L.The minimum detection limit was 1μg/L. The average recovery rate for urotropine was 81.67%- 101.67%.The method is simple, accurate and suitable for the identification and quantification. Key words： Ultra performance liquid chromatography- tandem mass spectrometry; dried beancurd sticks; urotropine ~ determination

关 键 词：超高效液相色谱-串联质谱法 腐竹 乌洛托品 检测 

分 类 号：TS207[轻工技术与工程—食品科学]