矢车菊-3-葡萄糖苷对环氧丙酰胺诱导的MDA-MB-231细胞氧化应激的保护作用  

Protective Role of Cyanidin 3-Glucoside on Glycidamide-induced Cell Damage in MDA-MB-231 Cells

作　　者：宋柬[1,2] 张莉[1] 赵婧[1] 胡小松[1] 陈芳[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院国家果蔬加工工程技术研究中心,北京100083 [2]山东省疾病预防控制中心食品与营养所,济南250014

出　　处：《中国食品学报》2014年第7期21-26,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(81102152);国家"973计划"项目(2012CB720805);新世纪优秀人才支持计划项目(NCET-12-0520)

摘　　要：从干预细胞氧化应激的角度,采用比色法、荧光检测及免疫分析的方法,考察不同浓度的矢车菊-3-葡萄糖苷对环氧丙酰胺引起的人类乳癌细胞MDA-MB-231毒性的影响。试验结果表明,与环氧丙酰胺单独处理的细胞相比,矢车菊-3-葡萄糖苷预处理后能明显提高细胞存活率,显著降低细胞内活性氧和丙二醛的生成量,而且抑制细胞内还原型谷胱甘肽含量的下降,提高γ-GCS蛋白表达量。矢车菊-3-葡萄糖苷能够保护MDA-MB-231细胞免受环氧丙酰胺引起的氧化应激。Acrylamide（AA） formed in carbohydrates-enriched foods during heating processing. AA has been categorized as a possible carcinogen. Glycidamide（GA）, a reactive epoxide, is the metabolite of AA in body and more toxic than AA itself. Cyanidin 3-glucoside（Cy-3-glu） is one of the natural antioxidants widely found in plants. In the present study, we investigated whether Cy-3-glu can decrease the GA-reduced cytotoxicity of human breast cancer MDA-MB-231 cells. Colorimetry assays, fluorescence detection and immunoblot analysis were performed. Results showed that Cy-3-glu significantly enhanced cell viability and reduced the generation of reactive oxygen species（ROS） and malondialdehyde（MDA） Moreover, the pretreatment with Cy-3-glu suppressed glutathione（GSH） delption and increased gamma-glutamylcysteine synthetase（γ-GCS） protein expression by GA. Cy-3-glu could present the protection against cell damage induced by GA in MDA-MB-231 cells.

关 键 词：矢车菊-3-葡萄糖苷 环氧丙酰胺 细胞损伤 活性氧 谷胱甘肽 

分 类 号：R737.9[医药卫生—肿瘤] O623.7[医药卫生—临床医学]