豆豉纤溶酶载纳米脂质体的制备与表征  

Preparation and Characterization of Douchi Fibrinolytic Enzyme Loaded Nanoliposomes

作　　者：张婵[1] 李小东[1] 王成涛[1] 

机构地区：[1]北京工商大学北京市食品添加剂工程技术研究中心食品质量安全北京实验室,北京100048

出　　处：《中国食品学报》2014年第7期32-38,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：教育部科学技术研究重点项目(211101);北京市教委面上项目(KM201110011001);北京市属高等学校创新团队建设与教师职业发展计划项目(2013113)

摘　　要：为提高豆豉纤溶酶口服生物利用度,建立了豆豉纤溶酶载纳米脂质体系统,并对其表征进行评价。采用硫酸铵梯度法制备的豆豉纤溶酶纳米脂质体的包封率为(52.74±4.24)%,粒径(72.04±31.2)nm,平均Zeta电位-44.2 mV,PdI 0.237。试验结果表明:豆豉纤溶酶纳米脂质体在体外稳定性好,在人工肠液中的释放符合一级动力学释放规律,豆豉纤溶酶在12 h后释放接近完全,无突释现象。豆豉纤溶酶及载酶纳米脂质体肠吸收液酶活性测定结果表明:豆豉纤溶酶及其载酶纳米脂质体在小肠均有吸收,吸收后仍具有纤溶活性。纳米脂质体可有效促进豆豉纤溶酶的吸收。In order to effectively improve the oral bioavailability of douchi fibrinolytic enzyme（DFE）, we established DFE loaded nanoliposomes oral drug absorption system in this study, and detected its characterization. DFE loaded nanoliposomes were prepared by ammonium sulfate gradient method. Its encapsulation efficiency, size, mean Zeta potential and polydispersity index（PdI） were（52.74±4.24）%,（72.04±31.2） nm,-44.2 mV and 0.237, respectively. DFE loaded nanoliposomes had steadiness in vitro. The release was fitted with fist order kinetic equation in artificial intestinal juice,and DFE was completely released in 12 h without burst effect. Activity measurement of DFE and intestinal solution of DFE loaded nanoliposomes were established. Both DFE and DFE loaded nanoliposomes could be absorbed by intestine and still active after absorbing.

关 键 词：豆豉纤溶酶 纳米脂质体 包封率 粒径大小 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ460.1[轻工技术与工程—食品科学与工程]