高效液相色谱测定脱脂豆粕中嘌呤含量  

Determination of Purines in Defatted Soybean by High Performance Liquid Chromatography

作　　者：崔素萍[1] 车康[1] 谢微[1] 崔丽琴[1,2] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江贝因美乳业有限公司,黑龙江绥化151400

出　　处：《中国食品学报》2014年第7期224-229,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省研究生创新科研项目(YJSCX2011-261HLJ)

摘　　要：为检测脱脂豆粕中嘌呤含量,利用高氯酸水解豆粕样品中的核酸,采用高效液相色谱法测定豆粕粉中嘌呤含量。测定结果表明:色谱柱为Ultimate AQ-C18(4.6 mm×250 mm,5μm);柱温25℃,进样量10μL,流速1.0mL/mim,紫外检测器波长254 nm;流动相为水、冰乙酸、四丁基氢氧化铵以997∶1.5∶1.5的比例混合,再与无水甲醇以99∶1混合。以此方法测定的腺嘌呤、鸟嘌呤、黄嘌呤、次黄嘌呤的精密度RSD分别为0.31%,0.27%,0.50%,0.41%;回收率分别为89.00%,86.90%,88.10%,81.30%。通过此方法测定脱脂豆粕粉中的嘌呤含量为253.0 mg/100g。In order to detect the purine contents of defatted soybean, HClO4 was used to hydrolyze nucleic acid of defatted soybean, the high performance liquid chromatography（HPLC） methods was developed. Results of the present study suggest that, the optimized chromatography conditions were as follows： Ultimate AQ-C18（4.6 mm×250 mm, 5 μm）;1.0% methomol of water： acetic acid： tetra butylammonium hydroxide（997∶1.5∶1.5）was as mobile phase at flow rate 1.0moL/min at 25 ℃ and a injection volume 10 μL. UV detector was employed at wavelength of 254 nm. The RSD of precision of Adenine, Guanine, Xanthine, Hypoxanthine were： 0.31%, 0.27%, 0.50%, 0.41%, respectively. Their average recoveries were 89.00%, 86.90%, 88.10%, 81.30%, respectively. The purine contents of defatted soybean was 253.0 mg/100 g detected by HLCP.

关 键 词：脱脂豆粕 嘌呤 高效液相色谱 回收率 精密度 

分 类 号：O657.72[理学—分析化学]