合成胞内多糖的发酵灵芝菌种及培养基的研究  

The Study of Ganoderma Lucidum Strains and Its Cultural Mediums for Intracellular Polysaccharide

作　　者：曾化伟[1,2,3] 郑惠华[1,2,3] 梁伟[1,2,3] 陈惠[1,2,3] 

机构地区：[1]江苏安惠生物科技有限公司,江苏南通226009 [2]中科院微生物研究所与江苏安惠生物科技有限公司药用菌联合实验室,江苏南通226009 [3]南通市药用菌研究重点实验室,江苏南通226009

出　　处：《食品与发酵科技》2014年第4期22-25,共4页Food and Fermentation Science & Technology

基　　金：国家863计划项目(2012AA021704);2013江苏省科技厅企业"博士集聚"计划

摘　　要：利用12株灵芝菌种和11个培养基配方,进行了液体摇瓶发酵试验,结果表明:不同灵芝菌种和培养基的多糖含量和菌体干重存在一定的差异。优选出可供生产的组合为菌株LZ-18和培养基K,多糖含量为8.51%,菌体干重为23.8g/L。进一步进行该菌种和培养基的500L发酵罐的中试培养试验,表明仅培养56h,多糖含量达到最高6.23%,此时的菌体干重为25.0g/L。The different effect of intracellular polysaccharide （IPS） content and dry cell weight （DCW） was investigated by flask cultivation with 12 Ganoderma Lucidum strains and 11 cultural mediums. When Strain LZ-18 with fermentation medium K were chosen as preferred results, IPS content of 8.51% and DCW of 23.8g/L were obtained. Subsequently, expanding culture in a 500-1 fermentor was performed under the preferred results, the highest IPS content （6.23%） and DCW （25.0g/L） were obtained at only 56h.

关 键 词：灵芝 胞内多糖 培养基 液体发酵 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]