茶树“龙井43"叶绿体基因组测序及其系统进化(英文)  

Entire chloroplast genome sequence of tea(Camellia sinensis cv.Longjing 43):a molecular phylogenetic analysis

作　　者：叶晓倩[1] 赵忠辉[1] 朱全武[1] 王营营[2] 林张翔[2] 叶楚玉[2] 樊龙江[2] 须海荣[1] 

机构地区：[1]浙江大学农业与生物技术学院茶叶研究所,杭州310058 [2]浙江大学农业与生物技术学院农学系/浙江省作物种质资源重点实验室,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2014年第4期404-412,共9页Journal of Zhejiang University:Agriculture and Life Sciences

摘　　要：利用高通量测序技术对中国茶树品种龙井43"叶绿体基因组的全序列进行测定,拼接补洞后再利用Sanger测序法对序列进行验证,最终得到龙井43"叶绿体基因组全序列(GenBank登录号:KF562708.1).该基因组大小为157 085bp,其中大单拷贝区的长度为86 642bp,小单拷贝区的长度为18 283bp,反向重复区的长度为26 080bp,共注释叶绿体基因134个.与韩国茶树品种叶绿体基因组序列比对,在编码区发现15个基因发生了非同义突变,在非编码区有100多个多态性位点,这些突变可作为茶树品种鉴定的DNA标记.同时,选取12个中国茶树品种,对其叶绿体基因组上的特异性片段(ycf1,psbA-trnH,psbK-psbI-psbI)进行测序比对并构建系统进化树.结果表明:12个茶树品种在进化树上分为2个亚群,其中凌云白毛茶"单独形成一支,其他11个品种形成一支;而11个品种中,龙井瓜子"龙井长叶"龙井圆叶"中茶102"形成一支,自展支持率为100%.表明利用叶绿体基因片段可有效区分茶树品种间的亲缘关系.Camellia sinensis cv . Longjing 43 is a domestic variety of tea species and an important economic crop in China . In this study , we developed a rapid method to get the chloroplast （cp） genome and sequenced the entire cp genome sequence of C . sinensis cv . Longjing 43 . The C . sinensis cv . Longjing 43 cp genome was 157 085 bp in length , which contained a large single-copy （ LSC , 86 642 bp） region , a small single-copy （ SSC , 18 283 bp） region , and two inverted repeat （ IR , each with a size of 26 080 bp） regions . With the cp genome of Korean C . sinensis cultivar as a reference , 134 chloroplast genes were successfully annotated . There were 15 genes with non-synonymous mutations in the coding region and more than 100 polymorphic sites in the non-coding region , which could be the DNA markers for the determination of different C . sinensis varieties . We also investigated the relationship of 12 C . sinensis varieties in China based on several cp genomic regions , which contain many variant sites . The result showed that these varieties were divided into two groups with Lingyunbaimaocha in one group and the other 11 in another group . Among the other 11 varieties , the Longjingchangye , Longjingyuanye , Longjingguazi , and Zhongcha 102 had a closer relationship and were formed into one cluster with 100% support rate , demonstrating the reliability of the method that used the cp genome sequences to investigate the genetic relationships .

关 键 词：龙井43 叶绿体基因组 高通量测序 亲缘关系 系统发育树 

分 类 号：S571.1[农业科学—茶叶生产加工] Q7[农业科学—作物学]