Target genes involved in antiproliferative effect of modified ginseng extracts in lung cancer A549 cells  

作　　者：Keun-Hong Kim Ilsan Choi Yeon-Weol Lee Chong-Kwan Cho Hwa-Seung Yoo Seung-Bae Lee Suk Ho Choi Ki-Rok Kwon Jun-Hyeog Jang 

机构地区：[1]East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University, Daejeon 302-122, Korea [2]Department of Biochemistry, lnha University School of Medicine, Incheon 400-712, Korea [3]Division of Animal Resources and Life Science, Sangji University, Wonju 220-702, Korea [4]Research Center of Pharmacopuncture Medicine, Korean Pharmacopuncture Institute, Seoul 157-801, Korea

出　　处：《Acta Biochimica et Biophysica Sinica》2014年第6期441-449,共9页生物化学与生物物理学报（英文版）

摘　　要：Lung cancer is the most common cancer and the leading cause of cancerrelated deaths. Panax ginseng has long been used to treat cancer and other diseases worldwide. Most of the pharmacological actions of ginseng are attribu ted to a variety of ginsenosides, which are often metabo lized by intestinal bacteria into more effective forms. In this study, we found that the antiproliferative activity of ginseng was increased after enzymatic processing of ginseng saponin （50% inhibitory concentration, 〉70μg/ml）. To elucidate the mechanism by which modified ginseng extract （MGX） induced cell death in human lung cancer cells, the gene expression profiles ofA549 cells regulated by MGX were assayed using Agilent PrimeView Human Gene Expression Arrays. The expression of 17 genes involved in the regulation of cell signaling, cell metabolism, transport, and cytoskeletonregulation was upregulated, whereas the expression of 16 genes implicated in invasion and metastasis and cellular metabolism was downregulated in MGX treated A549 cells. Moreover, nuclear staining with 4：6dia midino2phenyHndole revealed that MGX clearly caused nuclear condensation and fragmentation which are observed in apoptosis cell. These results elucidate crucial antieancer mechanisms of MGX and provide potential new targets for the assessment of anticancer activity of MGX.Lung cancer is the most common cancer and the leading cause of cancerrelated deaths. Panax ginseng has long been used to treat cancer and other diseases worldwide. Most of the pharmacological actions of ginseng are attribu ted to a variety of ginsenosides, which are often metabo lized by intestinal bacteria into more effective forms. In this study, we found that the antiproliferative activity of ginseng was increased after enzymatic processing of ginseng saponin （50% inhibitory concentration, 〉70μg/ml）. To elucidate the mechanism by which modified ginseng extract （MGX） induced cell death in human lung cancer cells, the gene expression profiles ofA549 cells regulated by MGX were assayed using Agilent PrimeView Human Gene Expression Arrays. The expression of 17 genes involved in the regulation of cell signaling, cell metabolism, transport, and cytoskeletonregulation was upregulated, whereas the expression of 16 genes implicated in invasion and metastasis and cellular metabolism was downregulated in MGX treated A549 cells. Moreover, nuclear staining with 4：6dia midino2phenyHndole revealed that MGX clearly caused nuclear condensation and fragmentation which are observed in apoptosis cell. These results elucidate crucial antieancer mechanisms of MGX and provide potential new targets for the assessment of anticancer activity of MGX.

关 键 词：GINSENG SAPONINS CANCER GINSENOSIDE APOPTOSIS 

分 类 号：Q255[生物学—细胞生物学] S853.7[农业科学—临床兽医学]