酶法转化L-谷氨酸生产α-酮戊二酸  

Enzymatic production of α-ketoglutaric acid by L-glutamate oxidase from L-glutamic acid

作　　者：牛盼清[1,2,3] 张震宇[2] 刘立明[1,2,3] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]江南大学食品微生物制造工程实验室,江苏无锡214122

出　　处：《生物工程学报》2014年第8期1318-1322,共5页Chinese Journal of Biotechnology

基　　金：江苏省科技支撑计划社会发展项目(No.BE2013612);中组部青年拔尖人才支持计划;江苏省杰出青年基金(No.BK2012002)资助~~

摘　　要：利用L-谷氨酸氧化酶(LGOX),对酶法转化L-谷氨酸生产α-酮戊二酸(α-KG)的工艺条件进行了研究。首先对野生菌链霉菌Streptomyces sp.FMME066进行亚硝基胍诱变,获得一株遗传性状稳定的突变株Streptomyces sp.FMME067;突变株在最优培养基(g/L):果糖10,蛋白胨7.5,KH2PO4 1,CaCl2 0.05条件下,LGOX酶活为0.14 U/mL。LGOX的生化特征为最适pH 8.5、温度35℃,Mn2+是激活剂。对LGOX转化L-谷氨酸生产α-KG的条件进行优化,在最优条件下转化24 h,α-KG产量为38.1 g/L,转化率为81.4%。研究结果为开发LGOX酶法转化生产α-KG的工业化奠定了坚实的基础。We produced α-ketoglutaric acid （α-KG） from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase （LGOX）. First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2＋ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions,the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.

关 键 词：L-谷氨酸氧化酶 生化特征 发酵优化 酶法转化 

分 类 号：TQ921[轻工技术与工程—发酵工程]