鱼油对母鼠及新生大鼠海马组织NR1、c-fos、CREB基因表达的影响  

Effect of fish oil on expression of NR1, c-fos and CREB in hippocampus of female rats and newborn rats

作　　者：岳崟 余诗婷 张继文[1] 孙宗毅[1] 王丽梅[1,2] 刘烈炬[1] 

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023 [2]农产品加工湖北省协同创新中心,湖北武汉430023

出　　处：《中国酿造》2014年第8期47-50,共4页China Brewing

基　　金：武汉轻工大学校立重点项目(2013d11);国家自然科学基金青年科学基金项目(31000772)

摘　　要：研究鱼油对母鼠与新生大鼠海马组织中NR1、CREB、c-fos基因表达的影响。将64只成年SD大鼠随机分成阴性对照组和鱼油低剂量[150 mg/(kg·d)]、中剂量组[300mg/(kg·d)]和高剂量组[600 mg/(kg·d)],连续饲养60 d,使用实时定量PCR技术检测母鼠以及新生大鼠海马组织N-甲基-D-天门冬氨酸受体亚基(NR1)、即刻早期基因(c-fos)、cAMP反应元件结合蛋白(CREB)mRNA的表达。结果表明,与阴性对照组相比,鱼油低剂量组和中剂量组的母鼠与新生大鼠海马组织中NR1、CREB、c-fos基因的mRNA表达量显著提升,鱼油高剂量组母鼠与新生大鼠海马组织中NR1、CREB、c-fos基因的mRNA的表达量没有明显变化。孕期和哺乳期的饮食中添加一定剂量的鱼油,能够提高母鼠与新生大鼠海马组织中NR1、CREB、c-fos基因的表达。To investigate the effect of fish oil on mRNA expression of N-methyl-D-aspartate receptor subunit （NR1）, immediate early gene c-fos and cAMP responsive element binding protein（CREB） in hippocampus of female rats and newborn rats, 64 adult SD rats were randomly divided in- to negative control group, low dosage fish oit group [150 mg/（kg·d）], mid dosage fish oil group [300 mg/（kg·d）] and high dosage fish oil group [600 mg/（kg·d）]. After continuously feeding for 60 d, Real-time quantitative PCR was used to test the mRNA expression ofNR1, c-fos and CREB in hippocampus of female rats and newborn rats. The results showed that compared with the negative control group, the mRNA expression of c-fos, CREB and NR1 in the hippocampus female rats and newborn rats with low dosage and mid dosage offish oil group increased obviously. But there was no significant difference in the mRNA expression of c-los, CREB and NR1 in the hippocampus female rats and newborn rats with high dosage fish oil group. The optimal dosage of fish oil supplementation during the prenatal and suckling period could increase the mRNA expression of c-fos, CREB and NR1 in hippocarnpus of female rats and newborn rats.

关 键 词：鱼油 即刻早期基因 CAMP反应元件结合蛋白 N-甲基-D-天门冬氨酸受体亚基 大鼠 

分 类 号：Q591.5[生物学—生物化学] TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]