间接ELISA检测不同贮存条件下花生中黄曲霉毒素B_1  

Determination of aflatoxin B_1 in peanut under different storage conditions by indirect ELISA

作　　者：陈彤[1] 王常青[1] 李小凡[1] 郝志萍[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《中国油脂》2014年第9期88-91,共4页China Oils and Fats

摘　　要：建立了一种利用间接ELISA检测花生中黄曲霉毒素B。的方法，同时考察了平衡相对湿度及贮存时间对花生中黄曲霉毒素B。（AFB1）含量的影响。结果表明：该方法的重现性好，AFB1回收率在95．20％-97．75％之间，线性范围为0．049～0．971μg／kg，最低检出限为O．029txg／kg；花生中AFB1的含量随平衡相对湿度的升高而增加，随贮存时间延长，其含量也不断增加。但平衡相对湿度低于57．6％时，即使贮存90d，花生中的AFBl含量仍低于国家食品安全标准限量（20μg／kg）。The method for determing aflatoxin B1 （AFB~）in peanut by indirect ELISA was established, and the effects of equilibrium relative humidity and storage time on the content of AFBl were studied. The re- sults showed that the method had good reproducibility, and the recovery rate, the linear range and the limit of detection were 95.20% -97.75% , 0. 049 -0. 971 μg/kg and 0. 029 μg/kg respectively; the content of AFB1 in peanut increased with equilibrium relative humidity rising and storage time prolonging, while the content of AFB1 was still below 20 Ixg/kg of the limitation of the national food safety standard, when peanut was stored for 90 d under the condition of the equilibrium relative humidity below 57.6%.

关 键 词：花生 AFB1 酶联免疫吸附法 相对湿度 平衡水分 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ646.1[轻工技术与工程—食品科学与工程]