酶解法辅助提取花椒油树脂的工艺优化  

Optimization of Extraction Process Conditions of Oleoresin from Zanthoxylum bungeanum by Enzymatic Method

作　　者：陶志杰[1] 王睿 王改玲[1] 许晖[1] 朱晓霞[1] 孙晓侠[1] 

机构地区：[1]蚌埠学院,安徽蚌埠233030 [2]成都市青白江区农村发展局,四川成都610300

出　　处：《绥化学院学报》2014年第9期150-153,共4页Journal of Suihua University

基　　金：安徽高校省级自然科学研究项目(KJ2013Z198);安徽省教育厅科研项目(KJ2009B076);安徽省高等学校省级质量工程项目(项目编号:20101094)

摘　　要：试验以纤维素酶法辅助优化提取花椒油树脂。通过利用单因素和正交试验分析,结果表明,酶解温度为影响提取效果的显著因子,优化后提取条件为酶用量2.0mg/100mL,酶解时间1.5h,酶解温度45℃,在该工艺条件下提取一次花椒油树脂的平均提取率为30.2%。验证试验表明,经酶解提取法提取花椒油树脂与未经酶解处理的乙醇浸提法相比,结果增长25.8%。The present study was conducted to optimize the extraction of oleoresin from Zanthoxylum bungeanum with cellulase. By using one-factor-at-a-time and orthogonal array design methods, the results showed that hydrolysis temperature was the significant factor, enzyme dose of 2.0 mg/100 mL, hydrolysis time of 1.5 h and hydrolysis temperature of 45℃. Under the optimum conditions, the extraction efficiency of oleoresin was 30.2%, 25.8%higher than the yield of ethanol extraction.

关 键 词：花椒 油树脂 纤维素酶 正交试验 

分 类 号：TS264.3[轻工技术与工程—发酵工程]