海带岩藻聚糖硫酸酯降解及基本结构分析  

Degradation and structure analysis of fucoidan extracted from Laminaria Japonica

作　　者：郭峰君[1] 胡靖[1] 赵雪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2014年第18期93-97,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(31371759)

摘　　要：用离子交换色谱法对海带岩藻聚糖硫酸酯粗糖进行分离纯化,然后采用铜-过氧化氢氧化降解法获得低分子量多糖,对降解条件和产物进行研究。得出结论:离子交换色谱中加入一定浓度氯化钠,可以获得高硫酸根和岩藻糖含量的岩藻聚糖硫酸酯。红外光谱和核磁共振分析确定硫酸根的位置在C2或C3上,糖构型属于α-D-吡喃糖。研究表明自由基氧化降解海带岩藻聚糖硫酸酯的降解条件与其硫酸根和岩藻糖含量有关,硫酸根含量越高,多糖越难以降解。最优降解条件:60℃下,500μL 20mg/mL糖溶液,加入0.0267mol/L乙酸铜,F3、F4加10μL 15%H2O2,F5加50μL15%H2O2,反应4h。采用PAGE凝胶电泳方法可以很好的分析氧化降解产物的分子量分布。To separate and purify fucoidan from Laminaria Japonica by anion-exchange chromatography. To obtain and analyze low molecular weight fucoidan by copper-hydrogen peroxide free radical degradation,High sulfate and fucose content fucoidan were obtained by certain concentration of sodium chloride from anionexchange column. Infrared spectrum method determined the position of the sulfated group was on C2 or C3. NMR further demonstrated the existence of α-D-pyranose. The molecular weight of fucoidan and low molecular weight fucoidan fractions were analyzed with PAGE electrophoresis. Result revealed that the content of sulfate group and fucose played an important role on the free radical degradation of fucoidan. The fucoidan fraction with high sulfate and fucose content was more difficult to be degraded. The optimal degradation conditions were obtained ：500μL 20mg/mL fucoidan solution,with 0.0267mol/L cupric acetate,10μ/L 15% H2O2 for F3,F4 and 50μL 15% H2O2 for F6,reacted for 4h at 60℃. Using the method of PAGE could analyze the distribution of the molecular weight of oxidative degradation products.

关 键 词：海带 岩藻聚糖硫酸酯 氧化降解 聚丙烯酰胺凝胶电泳 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]