响应面法优化蒸参水中挥发油提取条件及GC-MS分析  

Extraction conditions optimization of essential oil from steamed ginseng water by response surface methodology and GC-MS analysis

作　　者：牛春颖[1] 张凤清[1] 刁东梅 付彦臣 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012 [2]四平宏宝莱饮品股份有限公司技术部,吉林四平136000

出　　处：《食品工业科技》2014年第18期310-314,319,共6页Science and Technology of Food Industry

基　　金：吉林省医药产业发展专项资金(YYZX201130-2)

摘　　要：以红参加工副产物"蒸参水"为原料,采用溶剂提取法提取蒸参水中挥发油,在单因素基础上采用响应面法对提取条件进行优化,同时采用气相色谱-质谱法(GC-MS)对蒸参水挥发油进行成分分析。结果表明,以石油醚为提取溶剂的最佳提取工艺条件为:提取时间8h,提取温度80℃,料液比1∶5,在此最优提取条件下蒸参水挥发油的最高提取率达到0.51%。气相色谱-质谱分析结果表明蒸参水挥发油中主要成分为烷烃类(61.497%)、酯类(19.192%)、酸类(11.773%)、醛类(1.821%)、醇类(4.367%),还含有一组倍半萜化合物石竹烯和金合欢烯。The raw material was the steamed ginseng water （the byproducts of the red ginseng processing）,the essential oil was extracted from the steamed ginseng water by solvent extraction method,the extraction technology was optimized by response surface methodology on the basis of a single factor,the component of the essential oil extracted from the steamed ginseng water was also analyzed by GC-MS. The results showed that when petroleum ether was used as extraction solvent,the optimum extraction conditions were 8h（extraction time） ,80℃（extraction temperature） and 1：5（feed liquid ratio）,the extraction ratio of essential oil from steamed ginseng water reached 0.51% in this optimum extraction conditions. The GC-MS analysis showed that the main component of the essential oil contained alkanes （61.497%）, esters （ 19.192% ）, acids （ 11.773% ）, aldehydes （ 1.821%）, alcohol （4.367%） and a set of sesquiterpenes caryophyllene and farnesene.

关 键 词：蒸参水 挥发油 响应面法 气相色谱-质谱法(GC-MS) 

分 类 号：TS209[轻工技术与工程—食品科学]