Nocardia canicruria BF313催化甾体9α-羟基化发酵工艺优化  

Optimization of submerged fermentation condition for9α-hydroxylation of Steroid catalyzed by Nocardia canicruria BF313

作　　者：魏长龙[1,2] 赵树欣[1] 王珊[1,2] 史吉平[2] 张保国[2] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院上海高等研究院,上海201210

出　　处：《食品工业科技》2014年第18期320-323,337,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(41106125)

摘　　要：应用微生物转化的方法,以Nocardia canicruria BF313作为实验菌株,雄烯二酮为底物,研究了生物法制备9α-羟基雄烯二酮的新工艺。实验考察了转化培养基成分、投料方式、初始pH等因素对微生物转化生成9α-羟基雄烯二酮的影响。最终确定的发酵培养基为:15g/L葡萄糖,2g/L蛋白胨,5g/L酵母粉,1.5g/L硫酸亚铁。灭菌前调pH为7.0,以4%的接种量接种,预培养16h后以拟结晶方式投加底物,经过48h的转化,在投料浓度为10g/L情况下,底物转化率可高达97.2%,在投料浓度20g/L情况下,底物转化率也可达82.7%,投料浓度高于国际水平10g/L,具有极好的产业化前景。Screened the fermentation medium for 9α-hydroxyiation of androstenedione catalyzed by Nocardia canicruria BF313,and the reaction conditions,such as feeding mode,the initial pH were optimized ,the fermentation medium was finalized ： 15g/L glucose,2g/L peptone,5g/L yeast extract, 1.5g/L FeSO4. Adjusted to pH7.0 before sterilization,then inoculated with 4% of the inoculum,added the substrate with Pseudo-crystallo mode after 16h pre-culture. 48h after conversion,the substrate conversion rate was 97.2% when the concentration of the feeding was 10g/L. Under the feeding concentration 20g/L,the substrate conversion rate could reach 82.7%. Conversion efficiency was much higher than the international level ,so the industrial prospects would be great.

关 键 词：微生物转化 雄甾-4-烯-3 17-双酮 9α-羟基化 发酵条件 优化 

分 类 号：Q939.97[生物学—微生物学]