乳腺癌原发灶和淋巴结转移灶中VEGF、nm23的表达变化及意义  

Expression and significance of VEGF,nm23 in primary tumors and lymph-node metastases in breast cancer

作　　者：宁殿宾[1] 赵玉哲[1] 祁红辉[1] 

机构地区：[1]河北省人民医院,石家庄050051

出　　处：《山东医药》2014年第35期16-18,共3页Shandong Medical Journal

基　　金：河北省卫生厅重点课题(20090224)

摘　　要：目的：观察乳腺癌原发灶及淋巴结转移灶中血管内皮生长因子（VEGF）及转移抑制基因（m23）的表达变化及意义。方法选择2005年1～12月在我院行手术治疗且术前均未行抗癌治疗的60例乳腺癌患者，术后病理证实无同侧腋窝淋巴结转移24例（无转移组），伴有同侧腋窝淋巴结转移36例（转移组），采用免疫组化SP法检测乳腺癌原发灶和淋巴结转移灶中VEGF、nm23的表达，并分析VEGF与nm23的相关性。结果转移组原发灶中VEGF阳性表达率为91．7％，转移灶中为61．1％，无转移组VEGF阳性表达率为70．8％，转移组原发灶中VEGF阳性表达率明显高于转移灶及无转移组（P均＜0．05）。转移组nm23阳性表达率在原发灶中为16．7％，转移灶中为41．7％，无转移组nm23阳性表达率为45．8％，转移组原发灶中nm23阳性表达率明显低于转移灶及无转移组（P均＜0．05）。原发灶VEGF与nm23表达呈负相关（r＝-0．415，P＜0．05）。原发灶VEGF、nm23表达与淋巴结转移灶中VEGF、nm23表达无相关性。结论乳腺癌原发灶中VEGF表达明显高于、nm23表达明显低于淋巴结转移灶， VEGF、nm23与乳腺癌淋巴结转移密切相关，二者在乳腺癌发生和淋巴结转移中起拮抗作用。Objective To investigate the expression and significance of VEGF and nm23 in primary tumors and lymph-node metastases in breast cancer.Methods Totally 60 breast cancer patients who underwent surgery in our depart-ment during the year of 2005 and without anticancer therapy pre-surgery were selected.Postoperative pathology confirmed that there were 24 cases （ the group without metastasis） without lymph node metastasis at the ipsilateral axillary, and 36 ca-ses （the group with metastasis） with lymph node metastasis.SP immunohistochemistry was used to detect the protein ex-pressions of VEGF, nm23 and the relationship between VEGF and nm23 was analyzed.Results In the group with metas-tasis, the positive rate of protein VEGF in the primary tumor （91.7%） was higher than that in the metastases （61.1%）, P〈0.05.The positive rate of protein VEGF in the group with metastasis （91.7%） was higher than that without metastases （70.8%）, P〈0.05.In the group with metastasis, the positive rate of protein nm23 in the primary tumor （16.7%） was lower than that in the metastases （41.7%）, P〈0.05.The positive rate of protein nm23 in the group with metastasis （16. 7%） was lower than that without metastases （45.8%） , P〈0.05.The expressions of VEGF and nm23 were negative cor-related（r=-0.415, P〈0.05）.The expression of VEGF and nm23 in breast primary tumor were not associated with that of lymph-node metastases.Conclusions The expression of VEGF in the breast primary tumor is significantly higher than that in the lymph-node metastases, but the expression of nm23 in the breast primary tumor is significantly lower than that in the lymph-node metastases.VEGF and nm23 are closely related with lymph node metastasis of breast cancer.They have an antagonistic effect in the development of breast cancer and lymph node metastasis.

关 键 词：乳腺肿瘤 乳腺癌 血管内皮生长因子 转移抑制基因NM23 METASTATIC SUPPRESSOR gene NM23 

分 类 号：R737.9[医药卫生—肿瘤]