大孔树脂纯化脂溶性玉米黄色素  

Purification of Liposoluble Maize Yellow Pigment by Macroporous Resin

作　　者：李晓玲[1,2] 王世清[2] 王文亮[1] 弓志青[1] 靳琼[1] 孙华[1] 

机构地区：[1]山东省农业科学院农产品研究所,济南250100 [2]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《中国食品学报》2014年第9期164-169,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271963);山东省农业重大应用技术创新课题(鲁财农指[2011]39)

摘　　要：为探究脂溶性玉米黄色素的纯化方法,从NKA-9、XAD-7HP、D101、DA-201和聚酰胺吸附树脂5种层析树脂中选出DA201大孔吸附树脂,研究其对脂溶性玉米黄色素静态和动态吸附解吸性能,结果表明:DA201大孔树脂对脂溶性玉米黄色素的吸附4 h后达到平衡,饱和吸附量为0.0891 mg/g干树脂,最佳解吸剂为无水乙醇。玉米黄色素粗提浓缩液以2.5 mL/min流速上柱,吸附平衡后,以无水乙醇进行动态等度洗脱,研究其动力学过程。HPLC分析表明,动态纯化后玉米黄色素的质量分数从53.14%提高到86.64%。To investigate the optimum purification methi）d of maize yellow pigment, DA-201was selected from 5 kinds of macroporous resin including NKA-9, XAD-7HP, D101, DA201 and polyamide resin. The static-dynamic adsorption and desorption of maize yellow pigment by DA-201 were studied. Results showed that saturated adsorption capacity of DA-201 resin was 0.0891 mg/g dry resin and absolute ethyl alcohol was the optimal elution solution. The process of dynamic adsorption and desorption were also studied. With crude extraction＇s flow rate of 2.5 mL/min, the pigment was eluted by absolute ethyl alcohol when the adsorption reaction reached adsorption equilibrium. HPLC analysis indicated that mass concentration of liposoluble maize yellow pigment increased from 53.14% to 86.64% after the dynamic purification.

关 键 词：玉米黄色素 大孔树脂 吸附 解吸 等度洗脱 

分 类 号：TQ914.1[化学工程] TQ460.1