应用里氏木霉发酵制备纤维素酶固体曲的研究  

Study on the Fermentation of Cellulase Solid Koji by Using Trichoderma reesei

作　　者：张宁[1,2,3,4] 蒋剑春[1,2,3,4] 杨静[1,2,3,4] 卫民[1,2,3,4] 赵剑 童娅娟[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042

出　　处：《生物质化学工程》2014年第5期7-10,共4页Biomass Chemical Engineering

基　　金：国家林业局公益性行业专项资助项目(201004001);江苏省自然科学基金资助项目(BK2012515)

摘　　要：应用本实验室筛选得到的纤维素酶高产菌株里氏木霉(Trichoderma reesei)150-1-1发酵制备纤维素酶固体曲,通过优化固态发酵培养基组成及发酵条件,制备得到纤维素酶活力较高的固体曲及粗酶液。实验结果表明,在原料量为10 g,麸皮与秸杆粉质量比为4∶1,料液比为1∶2.5,发酵时间为120 h,发酵温度为31℃,发酵起始pH值为5.5的条件下,应用里氏木霉150-1-1发酵得到的纤维素酶固体曲酶活力达到423.6 U/g。The cellulase solid koji was fermented by using high yielding strain Trichoderma reesei 150-1-1 screened in our laboratory. Solid koji and crude enzyme with high cellulase activities were obtained by optimizing the medium components and fermentation conditions of solid fermentation. The results showed that the cellulase activities of solid koji reached 423. 6 U/g under the optimal conditions where the fermentation material was 10 g,the ratio of straw powder to wheat bran was 4：1,the ratio of materials to water was 1：2. 5,the fermentation reaction temperature was 31℃,the reaction time was 120 h,and the original pH was 5. 5.

关 键 词：里氏木霉 纤维素酶 固体曲 发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]