人子宫内膜癌中p57^(kip2)、Cyclin D1的表达及意义  

Expression and significance of p57^(kip2),Cyclin D1 in human endometrial carcinoma

作　　者：格桑志玛[1] 周正平[2] 罗祖强[1] 肖庆邦[1] 孙小杰[1] 

机构地区：[1]遵义医学院病理学教研室,遵义563099 [2]遵义医学院医学与生物学研究中心电镜室,遵义563099

出　　处：《临床与实验病理学杂志》2014年第9期975-978,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：贵州省教育厅自然科研基金(黔教科2010047);贵州省社发攻关项目[黔科合SY字(2013)3011]

摘　　要：目的探讨p57kip2、Cyclin D1在人子宫内膜癌发生、发展中的作用。方法选取子宫内膜样腺癌(endometrioid adenocarcinoma,EA)100例、子宫内膜上皮内瘤变(endometrial intraepithelial neoplasia,EIN)20例、子宫内膜增生性病变20例、增生期子宫内膜组织20例;选取不同子宫内膜细胞[高分化子宫内膜癌细胞(Ishikawa)、中分化子宫内膜癌细胞(JEC)、低分化子宫内膜癌细胞(KLE)及正常子宫内膜细胞(ESC)]进行细胞培养。应用免疫组化EliVision法检测不同子宫内膜组织中p57kip2、Cyclin D1蛋白的表达;Western blot法检测不同子宫内膜细胞中p57kip2、Cyclin D1蛋白的表达。结果 p57kip2蛋白在EIN组织中表达最高,在增生期子宫内膜、EA、子宫内膜增生性病变组织中表达逐渐降低,子宫内膜增生性病变与EIN组织中p57kip2蛋白表达差异有统计学意义(P<0.05)。Cyclin D1蛋白在EA组织中表达最高,在增生期子宫内膜组织中表达最低,在子宫内膜增生性病变组织、EIN组织中表达依次增高,差异均有显著性(P<0.01)。p57kip2、Cyclin D1蛋白在EA组织中的表达随着组织学分级的增高呈依次递减趋势,但仅p57kip2蛋白表达和组织学分级有关(P<0.05)。p57kip2蛋白在KLE中表达最高,在ESC中表达最低,两组相比差异有显著性(P<0.05);Cyclin D1在JEC、Ishikawa中的表达高于ESC,差异均有显著性(P<0.05)。结论 p57kip2、Cyclin D1均参与子宫内膜癌的发生、发展。Cyclin D1表达是子宫内膜癌发生的早期事件,可能还存在异常合成的p57kip2蛋白,其协同Cyclin D1促进子宫内膜的恶性转化。联合检测p57kip2、Cyclin D1在子宫内膜癌中的表达,对预测子宫内膜癌患者预后有一定的临床意义。Purpose To study the effects of p57kip2,Cyclin D1 on the carcinogenesis and progression of endometrial carcinoma. Methods 100 cases of endometrial adenocarcinoma（ EA）,20 cases of endometrial intraepithelial neoplasia（ EIN）,20 cases of hyperplasia lesion,20 cases of endometrial proliferative phase were selected. Different endometrial cells（ well-differentiated endometrial cancer cells Ishikawa,moderately differentiated endometrial cancer cells JEC,poorly differentiated endometrial cancer cells KLE and normal endometrial cells ESC） were cultured. Protein expression of p57kip2,Cyclin D1 was measured by immunohistochemical EliVision methods. The expression of p57kip2,Cyclin D1 protein in different endometrial cells was detected by Western blot. Results The highest expression of p57kip2 protein was in EIN,the higher expression was in endometrial proliferative phase,the low expression was in EA and the lowest expression was in hyperplasia lesions,but only the expression of p57kip2 protein in EIN was higher than that in hyperplasia lesions（ P〈0. 05）. The highest expression of Cyclin D1 protein was in EA and the lowest expression of Cyclin D1 protein was in endometrial proliferative phase,expression of Cyclin D1 protein increased gradually in hyperplasia lesions and EIN,p57kip2,Cyclin D1 protein expression in the tissue of EA increased along with the histological grade,all present decreasing trend,but only p57kip2 protein expression related to histological grade（ P〈0. 05）. The highest expression of p57kip2 protein was in KLE and the lowest expression of p57kip2 protein was in ESC,but only the expression of p57kip2 protein in KLE was higher than that in ESC（ P〈0. 05）. The expression of Cyclin D1 in JEC,Ishikawa higher than that in ESC（ P〈0. 05）. Conclusion p57kip2,Cyclin D1 are involved in the occurrence and development of endometrial carcinoma,Cyclin D1 is an early event in endometrial carcinoma,but there may also be have abnormal p57kip2 protein by synthesized,synergistically. Cyclin

关 键 词：子宫肿瘤 子宫内膜癌 子宫内膜样腺癌 P57^KIP2 CYCLIN D1 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]