乳酸乳球菌KLDS4．0325的糖代谢过程及乳酸生物合成途径的比较  

Carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis subsp. lactis KLDS4. 0325

作　　者：杨晓春[1] 王玉堂[1] 周颖[1] 高晓峰[1] 李柏良[1] 霍贵成[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《微生物学报》2014年第10期1146-1154,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31171717);国家“863计划”(2012AA022108);食品安全与营养协同创新中心中心项目~~

摘　　要：【目的】为了探究乳酸乳球菌乳酸亚种KLDS4.0325的碳水化合物利用能力和乳酸形成潜力。【方法】本文对该菌株进行了全基因组鸟枪法测序,并应用生物信息学方法对该菌株细胞外糖的转运、代谢及产酸途径涉及的一系列基因与其它9株参考菌株进行了比较分析。【结果】与参考菌株相比,该菌株基因组中具有较多涉及整个途径糖代谢途径的关键酶编码基因。【结论】该菌株在基因水平上表现出能够利用多种糖类物质来产乳酸的优良性状,是一株具有高产L-乳酸工业潜能的乳酸菌。[Objective]We aimed to study the carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis KLDS4. 0325. [Methods]Whole genome shot gun strategy was used for genome sequencing of strain L. lactis KLDS 4.0325. Then,using bioinformatics method,we compared a series of protein- coding genes involved in transporting extracellular carbohydrate,sugar metabolism and lactic acid biosynthesis of strain L. lactis KLDS4. 0325 with other 9reference strains. [Results]In L. lactis KLDS4. 0325 genome,where possesses more key enzyme coding genes related to the whole pathway of sugar metabolism than reference strains. [Conclusion〗 In gene level,therefore,strain L. lactis KLDS4. 0325 shows a remarkable characteristic by utilizing various sugar to produce lactic acid,is a lactic acid bacteria with industrial potential of high yield L-lactic acid.

关 键 词：乳酸乳球菌KLDS4.0325 全基因组测序 糖转运蛋白 糖代谢 乳酸合成 比较分析 

分 类 号：TS201.3[轻工技术与工程—食品科学] Q591.1[轻工技术与工程—食品科学与工程]