酱香型红心曲中产红色素功能菌株的分离筛选  

Isolation & Screening of Red Pigment-producing Strains from Jiangxiang Red-core Daqu

作　　者：朱德文[1] 蒋英丽[2] 高鸿[1] 黄玉霞[1] 王晗[1] 沈毅[2] 罗爱民[1] 

机构地区：[1]四川大学食品工程系,四川成都610065 [2]四川郎酒集团有限责任公司,四川古蔺618200

出　　处：《酿酒科技》2014年第10期27-31,共5页Liquor-Making Science & Technology

基　　金：四川省科技厅支撑计划(2011GZ0020);酿酒生物技术及应用四川省重点实验室开放基金项目(2012LF3010)

摘　　要：以酱香型红心曲为材料,采用平板分离法筛选出1株酵母菌和3株产红色素优良的霉菌。结合菌株形态学分析及ITS区基因序列对菌株进行鉴定,分别鉴定为粘红酵母属(Rhodotorula mucilaginosa)和红曲霉属(Monascus ruber)。进一步对酵母产红色素进行紫外波谱扫描和浓酸显色反应,初步判断为类胡萝卜素。并且通过高效液相色谱法从3株红曲霉中筛选出1株高产Monacolin K(397.1μg/g)菌株。A yeast strain and three red pigment-producing mold strains were isolated from red-core Daqu by plate separation. Through strain morphological analysis and ITS region phylogenetic analysis, those strains were identified as Rhodotorula mucilaginosa and Monascus ruber. Further,Rhodotorula mucilaginosa was treated by UV spectroscopy scanning and concentrated acid color reaction, and it was prelimilarily judged as carotenoids. Among the three red pigment-producing strains, a high yield strain Monacolin K was screened out by HPLC.

关 键 词：酱香型红心曲 粘红酵母属 红曲霉属 类胡萝卜素 Monacolin K 微生物 

分 类 号：TS261.1[轻工技术与工程—发酵工程]