液相色谱法测定加工食品中红木素和降红木素  

Determination of bixin and norbixin in processed foods with HPLC

作　　者：钱疆[1,2] 陈健[1,2] 黄杰[1,2] 杨方[1,2] 刘正才[1,2] 张玉燕[1,2] 薛方财 

机构地区：[1]福建出入境检验检疫局技术中心,福州350001 [2]福建省检验检疫技术研究重点实验室,福州350001

出　　处：《分析试验室》2014年第10期1206-1210,共5页Chinese Journal of Analysis Laboratory

基　　金：国家质量监督检验检疫总局标准项目(2012B135);福建检验检疫局科技项目(FK2012-03)资助

摘　　要：建立了加工食品中天然色素胭脂树橙主要色素成分红木素、降红木素的液相色谱检测方法。采用氨水乙醇提取样品中的色素,石油醚除脂肪后,调节酸度使用二氯甲烷反提取目标色素,后采用C18色谱柱分离,流动相采用乙腈：2%乙酸（75∶25,V/V）,458 nm波长光度测定。方法定量限为1.0 mg/kg,线性范围为1~100 mg/kg。在样品中做1.0,10,100 mg/kg三水平添加回收,降红木素、红木素回收率分别在83.2%~99.3%和84.6%~103%,RSD分别为0.90%~4.9%和1.7%~5.5%,并采用相对保留时间法来定性,吸光比值系数换算法来定量不同构型的红木素和降红木素。A method for the determination of bixin and norbixin, which are the principal color components of annatto, in processed foods was reported. The sample was extracted with ethanol basified with ammonia hydroxide, and defatted by petroleum ether. After being acidified with glacial acetic acid, the targets were liquid-liquid extracted with dichloromethane. The colors acetonitrile and 2% glacial acetic acid （75： 25, V/V）. wavelength of 458 nm. The linearities of norbixin and limits were 1.0 mg/kg. The spiked recoveries ranged standard deviations of 0. 90% - 4.9% and 1.7% - 5. and norbix were qualified with the method of relative absorption coefficient ratio. were separated on a Cls column with the mobile phase of The targets were detected by a UV-Vis detector at the bixin ranged from 1 to 100 g/kg, and the quantitative from 83.2% to 99. 3% and 84. 6% ~103% with the 5%, respectively. The different configurations of bixin retention time and quantified with method of the light

关 键 词：胭脂树橙 红木素 降红木素 天然色素 食品添加剂 

分 类 号：O657.7[理学—分析化学]