海洋细菌Renibacterium sp.QD1产壳聚糖酶发酵培养基的统计优化  

Statistical optimization of fermentation medium for Chitosanase production from marine bacteria Renibacterium sp.QD1

作　　者：马靖峰 郭晓凤[1] 刘欢[1] 邢培川[1] 于文功[1] 路新枝[1] 

机构地区：[1]中国海洋大学医药学院、海洋药物教育部重点实验室、山东省糖科学与糖工程重点实验室,山东青岛266003

出　　处：《食品工业科技》2014年第21期146-149,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(41376144);国家高技术研究发展计划(863计划)(2011AA09070304);海洋公益性行业科研专项(201105027)(201005024);国家科技支撑计划(2013BAB01B02)

摘　　要：目的:通过对发酵培养基的统计优化提高海洋细菌Renibacterium sp.QD1壳聚糖酶的产量。方法:通过Plackett-Burman实验筛选影响壳聚糖酶产量的显著性因素。在此基础上利用最陡爬坡路径逼近最大响应区域,利用响应面法进行回归分析。结果:确定了影响壳聚糖酶产量的3个显著因素,其最佳浓度分别是壳聚糖14.23g/L、酵母粉4.72g/L、p H6.42。经模型验证,预测值与验证实验平均值接近。结论:在最优培养基中壳聚糖酶活力达到608.11U/m L,比优化前提高了52.03%。Objective：Using statistical optimization method to improve the yield of chitosanase from marine bacterium Renibacterium sp. QD1. Methods： Firstly, significant influencing factors were identified by two-level Plackett- Burman design（ PBD）.Then the path of the steepest ascent was utilized to approach the optimal region of the significant factors. Optimal concentrations of the medium were confirmed by response surface methodology. Results：Three elements were identified as the significant factors, and the optimal concentration was 14.23g/L for chitosan,4.72g/L for yeast and 6.42 for initial pH. Checking experiment results indicated that the maximum predicted value was in good agreement with mean of measured value.Conclusion：The yield of chitosanase under the optimal medium reaches 608.11U/mL of fermentation supernatant ,which was .52.03% higher than before the optimization.

关 键 词：Renibacterium sp.QD1 壳聚糖酶 PLACKETT-BURMAN设计 响应面分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]