桑黄胞外多糖发酵条件优化研究  

Experiment on Fermentation Technology for Production of Extracellular Polysaccharides from Phellinus igniarius

作　　者：程俊文[1] 侯寒黎 邹景泉[1] 贺亮[1] 李肖娟 

机构地区：[1]浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室,浙江杭州310023 [2]萧山出入境检验检疫局,浙江杭州311208 [3]杭州雪域生物技术有限公司,浙江杭州311200

出　　处：《浙江林业科技》2014年第5期16-20,共5页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省科技厅院所专项(2012F30037);浙江省科技厅院所专项(2014F50018);杭州市农业科研攻关专项(20120232B48)

摘　　要：在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化桑黄(Phellinus igniarius)液体发酵产胞外多糖的发酵工艺条件,结果表明:桑黄胞外多糖的最佳液体发酵培养条件为葡萄糖15.82 g/L,玉米粉10.51 g/L,豆饼粉9.55 g/L;此条件下的验证试验表明,此时胞外多糖的得率为461.72 mg/L,与理论值基本吻合。Analysis of three-level Box-Behnken factorial design with response surface methodology （RSM） was conducted on fermentationtechnology for extracellular polysaccharides （EPS） production from Phellinus igniarius based on single factor experiment. The result showed that theoptimal fermentation conditions were as follows： glucose 15.82 g/L, corn meal 10.51 g/L and soybean cake powder 9.55 g/L. Verification testdemonstrated that the average yield of EPS was 461.72 mg/L, similar to theoretical value of 476.99 mg/L.

关 键 词：桑黄 发酵 胞外多糖 Box-Behnken 响应面 

分 类 号：S759.81[农业科学—森林经理学]