松仁蛋白酶解液体外抗氧化活性分析  

In vitro antioxidant activity of pine kernel protein hydrolysate

作　　者：林秀芳[1] 吴晓红[2] 赵梓辰[1] 徐冰心[1] 高小棠[1] 刘敏[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]东北林业大学交通学院,哈尔滨150040

出　　处：《中国油脂》2014年第11期29-31,共3页China Oils and Fats

基　　金：中央高校基本科研业务经费专项(DL11BA13);黑龙江省博士后研究项目(LBH-Z11269);东北林业大学大学生创业训练项目(201310225080)

摘　　要：采用两种胃肠消化酶(胃蛋白酶、胰蛋白酶)对松仁蛋白进行酶解实验,研究其胃蛋白酶酶解液、胰蛋白酶酶解液和胃蛋白酶-胰蛋白酶分步酶解酶解液的体外抗氧化活性。结果表明:胃蛋白酶酶解30 min的酶解液具有最强体外抗氧化活性,·OH清除率为67.43%,O-2·清除率为55.66%;胰蛋白酶酶解60 min的酶解液具有最强清除·OH能力,·OH清除率为68.79%;胰蛋白酶酶解120 min的酶解液具有最强清除O-2·能力,O-2·清除率为39.20%;胃蛋白酶-胰蛋白酶分步酶解松仁蛋白酶解液在酶解240 min时,具有最强的体化抗氧化活性,此时·OH清除率为79.11%,O-2·清除率为68.99%。Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes （pepsin and trypsin ）, and in vitro antioxidant activities of enzymatic hydrolysates of pine kernel protein obtained by hydrolysis of pepsin, trypsin and pepsin - trypsin were studied. The results showed that after enzymatic hydrolysis for 30 min, the pepsin enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity, and the scavenging rates on · OH radical and 02- ~ radical were 67.43% and 55.66% respectively; the trypsin enzymatic hydrolysate of pine kernel protein had the strongest scavenging capacity on · OH radical after enzymatic hydrolysis for 60 min,and the scavenging rate on · OH radical was 68.79% ,while it had the strongest scavenging capacity on O2^-· radical after enzymatic hydrolysis for 120 min, and the scavenging rate on O2^-· radical was 39.20% ; pepsin - trypsin step - by - step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min, and the scavenging rates on · OH radical and O2^-· radical were 79.11% and 68.99% respectively.

关 键 词：松仁蛋白 酶解液 胃蛋白酶 胰蛋白酶 抗氧化活性 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ936[轻工技术与工程—食品科学与工程]