野生蓝莓提取物对四种致病菌的抑制作用  

Antibacterial Effects of Chinese Wild Blueberry Extracts on Four Pathogenic Bacteria

作　　者：姜文洁[1] 孙晓红[1] 朱颖[1] 杨晗[1] 赵勇[1] 吴启华 潘迎捷[1] 

机构地区：[1]上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海201306 [2]美国缅因大学食品与农业学院,缅因04469

出　　处：《天然产物研究与开发》2014年第11期1884-1888,共5页Natural Product Research and Development

基　　金：上海市科技兴农重点攻关项目(2014-3-5);上海海洋大学科技发展专项基金项目(A2-0209-14-200061);上海市科委工程中心建设(11DZ2280300)

摘　　要：本研究选用中国野生蓝莓,研究了其提取物对铜绿假单胞菌、单增李斯特菌、金黄色葡萄球菌与副溶血性弧菌等四种致病菌的抑制作用,分别采用试管二倍稀释法和平板涂布法测定了野生蓝莓提取物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明,野生蓝莓提取物对四种致病菌均有一定的抑制作用,对铜绿假单胞菌、单增李斯特菌、金黄色葡萄球菌与副溶血性弧菌的MIC分别为62.5、250、500 mg/m L与31.25 mg/m L,MBC分别为250、250、500与125 mg/m L。本研究首次报道了中国野生蓝莓对这四种致病菌的抑制作用,为中国野生蓝莓的开发和利用提供了依据。The antibacterial effects of Chinese wild blueberries extracts on 4 pathogenic bacteria,Pseudomonoa aeruginosa, Listeria monocogenes,Staphylococcus aureus and Vibrio parahaemolyticus, were investigated. The testing tube double dilution method and the spread plate method were used to determine minimal inhibitory concentration （MIC） and mini- mum bactericidal concentration （MBC）. The results showed that Chinese wild blueberry extracts had antibacterial effects on four investigated pathogenic bacteria. The MIC of wild blueberry extracts against P. aeruginosa, L monocytogenes,S. aureus and K parahaemolyt/cus were 62.5,250,500 mg/mL and 31.25 mg/mL, respectively. The MBC of wild blueberry extracts against P. aeruginosa, L. monocytogenes , S. aureus , and V. parahaemolyticus were 250,250,500 mg/mL and 125mg/mL,respeetively. This was the first study that chinese wild blueberry has antibacteridal effects the antibacterial effects of Chinese on the four pathogenic bacteria. The results will benefit future application of Chinese wild blueberry.

关 键 词：野生蓝莓 提取物 抑菌能力 二倍稀释法 

分 类 号：Q936[生物学—微生物学] TS202.3[轻工技术与工程—食品科学]