化学计量法结合气相色谱检测米糠油掺伪棉籽油  

Chemometrics approach applied to GC date for determination of cottonseed oil in rice bran oil

作　　者：喻凤香[1] 魏小军[1] 邓育平[1] 肖艳 

机构地区：[1]湖南生物机电职业技术学院,湖南长沙410127 [2]青岛科标化工分析检测有限公司,山东青岛266000

出　　处：《食品工业科技》2014年第23期271-274,281,共5页Science and Technology of Food Industry

基　　金：国家十二五科技支撑计划(2012BAD34B02);湖南省科技厅农业支撑计划(2014NK3058);湖南省教育厅基金(13C518);湖南省社科院基金(2013ZZ101)

摘　　要：为研究米糠油掺混棉籽油的快速定性定量检测方法,采用毛细管气相色谱法测定米糠油掺不同比例棉籽油的脂肪酸含量。将C10∶0、C14∶0、C16∶0、C16∶1、C17∶0、C18∶0、C18∶1、C18∶2、C18∶3、C20∶0、C20∶1、C22∶1的含量作为变量,聚类分析结果表明米糠油掺棉籽油5%以上能正确进行辨别,判别分析结果表明米糠油掺棉籽油20%以上能进行正确辨别,建立了基于特征脂肪酸变化的掺伪量计算一元线性模型YC14∶0=0.004X+0.3168(R2=0.9813),及基于指纹图谱相似度的掺伪量计算模型Y=-240.52X5+677.8X4-697.92X3+312.09X2-66.998X+99.97(R2=0.9993)。方法重复性好,准确度高,误差5%以下。To establish a rapid qualitative and quantitative detection model of cottonseed oil adulterated into rice bran oil（RBO） ,unadulterated RBO and adulterated RBO with different proportion cottonseed oil were detected by capillary gas chromatography.Contents of C10：0,C14：0, C16：0, C16：1, C17：0, CI8：0, C18：1, CI8：2, C18：3, C20：0, C20：1,C22：1 were made as variables.Qualitative detection was made by using cluster analysis and discriminant analysis.Result：Cluster analysis was able to made correct identify when RBO mixing cottonseed oil by more than 5% and discriminant analysis more than 20%.Quantitative detection models Yc14：0 = O.O04X ＋ 0.3168 （ R^2 = 0.9813） based on characteristic fatty acids and Y = -240.52X^5 ＋ 677.8X^4-697.92X^3 ＋ 312,09X^2-66.998X ＋ 99.97 （ R2 = 0.9993） based on fin clerprint similarity were built.The model had good repeatability, high accuracy with error less than 5%.

关 键 词：米糠油 棉籽油 气相色谱 判别分析 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]