采用GC测定调制乳中4种植物甾醇的检测方法  

Determination of Four Types Plant Sterol in Modified Milk with Gas Chromatography

作　　者：闫宁环[1] 陈伟[2] 钱文涛[2] 季国志[2] 李洪亮[2] 母智深[2] 

机构地区：[1]内蒙古化工职业学院,内蒙古呼和浩特011503 [2]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011500

出　　处：《食品研究与开发》2014年第19期103-105,共3页Food Research and Development

摘　　要：对调制牛乳中植物甾醇的测定方法进行了研究。样品在2 mol/L乙醇氢氧化钾60℃超声水浴处理15 min后，在60℃水浴中处理45 min进行皂化处理，正庚烷萃取后，再通过吡啶及MSTFA，在80℃水浴保温10 min进行衍生化。处理好的样品利用气相色谱法进行测定。结果表明：按照该法，测定的调制牛乳中四种植物甾醇的含量为170 mg/100 g，标准品的回收率（以豆甾醇记）为86.59%～103.09%，此法操作简单，灵敏度高。To research the sensitive method of determine the content of plant sterol in modified milk. Samples was disposed with hydroxide ultrasonic for 15 min in 2 mol/L ethanol potassium, and stayed it in 60℃water bath for 45 min,then extracting Plant sterol from liquid Heptanes,and then deriving through the Pyridine and MSTFA in 80℃water bath for 10 min. Finally, the sample was determined by Gas Chromatography .With this method, the total content of plant sterols was 170 mg/100 g, the average recovery of the standard （stigma sterol）was 86.59%-103.09%. This method was simple and accurate with high sensitivity.

关 键 词：调制乳 Β-谷甾醇 菜籽甾醇 菜油甾醇 豆甾醇 高效气相色谱 

分 类 号：O657.71[理学—分析化学] TS252.7[理学—化学]