高压脉冲电场对大肠杆菌噬菌体灭活效果初探  

Pulsed Electric Field Treatment for Inactivation of Escherichia coli Phage

作　　者：应南娇[1] 付荣[1] 杨勇[1] 章娟[1] 

机构地区：[1]杭州电子科技大学生命信息与仪器工程学院,浙江杭州310018

出　　处：《核农学报》2014年第8期1440-1445,共6页Journal of Nuclear Agricultural Sciences

基　　金：浙江省重大科技专项(2012C030);浙江省教育厅科研项目(Y201327362);浙江省自然科学基金(Y2100002)

摘　　要：为探讨高压脉冲电场（pulsed electric field,PEF）在噬菌体污染治理方面的工业应用,研究了高压脉冲电场对λ噬菌体、ΦX174噬菌体以及宿主菌E.coli MV1184的灭活效果。采用不同电场强度E,脉冲个数N和脉冲宽度г体系对λ噬菌体、ΦX174噬菌体以及λ噬菌体和E.coli MV1184混合悬液进行处理。结果表明在输出电压1~10kV,脉冲宽度1~10μs范围内,灭活效率随着脉冲数的增加而提高。PEF对噬菌体的灭活效率并不依赖于噬菌体的颗粒结构,但噬菌体颗粒结构对脉冲宽度г灭活效率有影响。在噬菌体与宿主菌E.coli MV1184的混合悬液中,λ噬菌体对PEF的灭活作用更加敏感,失活率显著高于E.coli MV1184。高压脉冲电场可以用于常温下溶液中噬菌体的灭活处理并达到消毒水平,是一种高效的噬菌体灭活方法。In order to investigate the pulsed electric field for inactivation of contaminated bacteriophage in food and chemical industry, the survival ratios of k phage, ФX174 phage and E. coli MV1184 were detected by PEF treatment. Three PEF system parameters, including electric field intensity, pulse number and pulse width, were crucially contributed to the inactivation results of k phage, ФX174 phage and E. coli MV1184. Phage titer was measured by the plaque forming unit method, and cells were observed with a gold field emission scanning electron microscopy. The survival ratios of the two bacteriophages decreased significantly depending on treatment time when electric field intensity set between 1 to 10kV, and pulse width set between 1 to 10μs. The results showed that inactivates bacteriophages independence of particle structures, although the structural differences had an impact on the inactivation efficiency. Bacteriophage is more sensitive to PEF treatment than E. coli cell by using mixture liquid. This study demonstrated that PEF treatment could inactivate E. coli phage in high efficiency at room temperature. PEF treatment could be applied for the phage -free fermentation process in food and chemical industry.

关 键 词：大肠杆菌 高压脉冲电场 灭活 噬菌体 

分 类 号：TS201.3[轻工技术与工程—食品科学]